Nitric oxide destabilizes pias3 and regulates sumoylation
文献类型：期刊论文
| 作者 | Qu, Jing; Liu, Guang-Hui1; Wu, Kaiyuan; Han, Peiwei; Wang, Peng; Li, Jiangmei; Zhang, Xu; Chen, Chang |
| 刊名 | Plos one
 |
| 出版日期 | 2007-10-31 |
| 卷号 | 2期号:10页码:9 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0001085 |
| 通讯作者 | Liu, guang-hui(ghliu@scripps.edu) |
| 英文摘要 | Small ubiquitin-related protein modifiers (sumo) modification is an important mechanism for posttranslational regulation of protein function. however, it is largely unknown how the sumoylation pathway is regulated. here, we report that nitric oxide (no) causes global hyposumoylation in mammalian cells. both sumo e2 conjugating enzyme ubc9 and e3 ligase protein inhibitor of activated stat3 (pias3) were targets for s-nitrosation. s-nitrosation did not interfere with the sumo conjugating activity of ubc9, but promoted pias3 degradation by facilitating its interaction with tripartite motif-containing 32 (trim32), a ubiquitin e3 ligase. on the one hand, no promoted trim32-mediated pias3 ubiquitination. on the other hand, no enhanced the stimulatory effect of pias3 on trim32 autoubiquitination. the residue cys459 of pias3 was identified as a target site for s-nitrosation. mutation of cys459 abolished the stimulatory effect of no on the pias3-trim32 interaction, indicating a requirement of s-nitrosation at cys459 for positive regulation of the pias3-trim32 interplay. this study reveals a novel crosstalk between s-nitrosation, ubiquitination, and sumoylation, which may be crucial for no-related physiological and pathological processes. |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000207458700004 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381779 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Guang-Hui |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Jing,Liu, Guang-Hui,Wu, Kaiyuan,et al. Nitric oxide destabilizes pias3 and regulates sumoylation[J]. Plos one,2007,2(10):9. |
| APA | Qu, Jing.,Liu, Guang-Hui.,Wu, Kaiyuan.,Han, Peiwei.,Wang, Peng.,...&Chen, Chang.(2007).Nitric oxide destabilizes pias3 and regulates sumoylation.Plos one,2(10),9. |
| MLA | Qu, Jing,et al."Nitric oxide destabilizes pias3 and regulates sumoylation".Plos one 2.10(2007):9. |
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来源：中国科学院大学
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