Increased activity of the non-regulated enzymes fructose-1,6-bisphosphate aldolase and triosephosphate isomerase in anabaena sp strain pcc 7120 increases photosynthetic yield
文献类型：期刊论文
| 作者 | Ma, Weimin; Wei, Lanzhen; Wang, Quanxi; Shi, Dingji; Chen, Haibao |
| 刊名 | Journal of applied phycology
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 19期号:3页码:207-213 |
| 关键词 | Anabaena sp Strain pcc 7120 Enzyme activity Fructose-1,6-bisphosphate aldolase Photosynthetic yield Triosephosphate isomerase |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-006-9125-8 |
| 通讯作者 | Shi, dingji(cyanoshi@163.com) |
| 英文摘要 | Non-regulated enzymes in the calvin cycle are generally presumed to be less important for the regulation of photosynthetic yield. here, to investigate the relationship between the activity of non-regulated enzymes and photosynthetic yield, two non-regulated enzymes in the calvin cycle-a rice cytosolic fructose-1,6-bisphosphate aldolase (fba) and a spinach chloroplast triosephosphate isomerase (tpi)-were cloned and co-expressed in cells of the cyanobacterium anabaena sp. strain pcc 7120. the activity of fba and tpi and the photosynthetic yield reflected by photosynthetic o-2 evolution and cell dry weight were measured and compared between wild-type and transgenic cells. our results demonstrated that the activity of fba and tpi were increased in transgenic cells relative to wild-type cells, and that activity was further increased in a transgenic strain harboring two sets of fba-tpi tandem genes relative to cells containing one copy of the fba-tpi tandem gene. the increased activity of fba and tpi in anabaena sp. strain pcc 7120 increased photosynthetic yield, with increased activity levels correlating closely with the degree of changes in photosynthetic yield. this implies that the photosynthetic yield is limited by the activity of the non-regulated enzymes fba and tpi, and that the endogenous activity of non-regulated enzymes is not sufficient to increase photosynthetic yield. we discuss the various roles of fba and tpi, and regulated and non-regulated enzymes, in modulating photosynthetic yield. |
| WOS关键词 | ENHANCES PHOTOSYNTHESIS ; POTATO PLANTS ; CO2 FIXATION ; GROWTH ; GENE ; CYANOBACTERIUM ; COEXPRESSION ; EXPRESSION ; PCC7120 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000246407100002 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381864 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shi, Dingji |
| 作者单位 | 1.Shanghai Normal Univ, Coll Life & & Environm Sci, Shanghai 200234, Peoples R China 2.Chinese Acad Sci, Inst Bot, Lab PHotosynth, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol,Nat Lab Plant Mol Genet, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Weimin,Wei, Lanzhen,Wang, Quanxi,et al. Increased activity of the non-regulated enzymes fructose-1,6-bisphosphate aldolase and triosephosphate isomerase in anabaena sp strain pcc 7120 increases photosynthetic yield[J]. Journal of applied phycology,2007,19(3):207-213. |
| APA | Ma, Weimin,Wei, Lanzhen,Wang, Quanxi,Shi, Dingji,&Chen, Haibao.(2007).Increased activity of the non-regulated enzymes fructose-1,6-bisphosphate aldolase and triosephosphate isomerase in anabaena sp strain pcc 7120 increases photosynthetic yield.Journal of applied phycology,19(3),207-213. |
| MLA | Ma, Weimin,et al."Increased activity of the non-regulated enzymes fructose-1,6-bisphosphate aldolase and triosephosphate isomerase in anabaena sp strain pcc 7120 increases photosynthetic yield".Journal of applied phycology 19.3(2007):207-213. |
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来源：中国科学院大学
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