Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro
文献类型：期刊论文
| 作者 | Xiong, Chuannan1,3; Wu, Haige1,2; Wei, Peng1,3; Pan, Ma1,3; Tuo, Yaqin1; Kusakabe, Isao4; Du, Yuguang1 |
| 刊名 | carbohydrate research
 |
| 出版日期 | 2009-10-12 |
| 卷号 | 344期号:15页码:1975-1983 |
| 关键词 | Deacetylated chitohexaose Angiogenesis ECV304 Vascular endothelial growth factor Urokinase plasminogen activator |
| ISSN号 | issn: 0008-6215 |
| 产权排序 | 1;1 |
| 通讯作者 | 杜昱光 |
| 英文摘要 | this study was performed to demonstrate the effects of deacetylated chitohexaose (hexamer) separated from a chitooligosaccharide (cos) mixture on tumor angiogenesis and its mechanism of action. five fractions from dimer to hexamer were separated by a linear gradient solution of hcl on a cation-exchange resin. then hcl was removed from the fractions by a charcoal column. the purity of the five fractions was analyzed by hplc and the molecular masses were analyzed by maldi-tofms. the hexamer expressed an inhibitory influence on cam angiogenesis in a dose-dependent manner at concentrations of 6.25-50 mu g/egg. on further investigation, we found that the hexamer had no toxic effect on normal ecv304 cells, but could inhibit the proliferation and migration of tumor-induced ecv304 cells in a dose-dependent manner. the mechanism was demonstrated through the detection of mrna expression of vegf, mmp-9, timp-1, timp-2 and upa by rt-pcr, which showed that the hexamer down-regulated the vegf and upa mrna expressions in ecv304 cells, but up-regulated the timp-1 mrna expression. crown copyright (c) 2009 published by elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemistry & molecular biology ; chemistry, applied ; chemistry, organic |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | endothelial growth-factor ; chick chorioallantoic membrane ; matrix metalloproteinases ; phytophthora-capsici ; tumor angiogenesis ; anti-angiogenesis ; oligochitosan ; cells ; induction ; strategies |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000271043300007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101905]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Univ, Bioengn Coll, Dalian 116622, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 305, Japan |
| 推荐引用方式 GB/T 7714 | Xiong, Chuannan,Wu, Haige,Wei, Peng,et al. Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro[J]. carbohydrate research,2009,344(15):1975-1983. |
| APA | Xiong, Chuannan.,Wu, Haige.,Wei, Peng.,Pan, Ma.,Tuo, Yaqin.,...&Du, Yuguang.(2009).Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro.carbohydrate research,344(15),1975-1983. |
| MLA | Xiong, Chuannan,et al."Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro".carbohydrate research 344.15(2009):1975-1983. |
入库方式： OAI收割
来源：大连化学物理研究所
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