Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry
文献类型：期刊论文
| 作者 | Yang, Li1; Wang, Yun1; Wang, Longxing2; Xiao, Hongbin2; Wang, Zhengtao1; Hu, Zhibi1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-03-13 |
| 卷号 | 1216期号:11页码:2098-2103 |
| 关键词 | Iridoid glycosides Longdan Xiegan Decoction Gentianae Radix Gardeniae Fructus High-performance liquid chromatography Electrospray ionization mass spectrometry Multiple reaction monitoring (MRM) |
| 产权排序 | 2;3 |
| 通讯作者 | zhengtao wang |
| 英文摘要 | longdan xiegan decoction (lxd) is a formulated preparation composed of 10 ingredient herbs, with iridoids as the main bioactive components. in this study, a rapid, simple and reliable method of simultaneous determination of four iridoid glycosides in lxd using high-performance liquid chromatography (hplc) coupled with electrospray ionization mass spectrometry (ms) was first developed and validated. the four iridoid glycosides references were isolated from lxd extract and purified using a preparative hplc chromatography. the sample preparation for quantification comprised of a simple ultrasonic extraction and the satisfactory chromatographic separation of the four structurally similar iridoid glycosides was effected in less than three minutes on a capcell pak c(18) mgii column (3 mu m, 100 mm x 2.0 mm), using an elution system of 10% methanol and their concentrations in different batches of lxd and ingredient herbs were simultaneously determined by hplc-ms/ms using a multiple reaction monitoring (mrm) mode. the method was validated with respect to the overall intra- and inter-day variation (rsd less than 8%) and the limits of quantification for the four iridoid glycosides were 35, 20. 37 and 33 ng/ml, respectively. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | diode-array detection ; quantitative-determination ; fingerprinting analysis ; secoiridoid glucosides ; hplc ; spectroscopy ; geniposide ; extracts ; plants ; esi/ms |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000264226500015 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101917]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Key Lab Standardizat Chinese Med, Minist Edu, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Li,Wang, Yun,Wang, Longxing,et al. Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry[J]. journal of chromatography a,2009,1216(11):2098-2103. |
| APA | Yang, Li,Wang, Yun,Wang, Longxing,Xiao, Hongbin,Wang, Zhengtao,&Hu, Zhibi.(2009).Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry.journal of chromatography a,1216(11),2098-2103. |
| MLA | Yang, Li,et al."Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry".journal of chromatography a 1216.11(2009):2098-2103. |
入库方式： OAI收割
来源：大连化学物理研究所
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