A new type of ca2+ sensor based on direct electrochemical capacitance method
文献类型：期刊论文
| 作者 | Qi Bin; Yang Xiu-Rong |
| 刊名 | Chinese journal of analytical chemistry
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 35期号:2页码:196-200 |
| 关键词 | Calmodulin Calcium ion Sensor Electrochemical capacitance Molecular recognization |
| ISSN号 | 0253-3820 |
| 通讯作者 | Yang xiu-rong(chemyang@ciac.jl.cn) |
| 英文摘要 | First, 16-mercaptohexaclecanoic acid was self-assembled onto the gold electrode surface via au-s covalent bond. the calmodulin was covalently immobilized onto the 16-mercaptohexadecanoic acid monolayer under the activation of edc and nhs. thus a new type of capacitance ca2+ sensor was constructed. the equivalent circuit analysis of the testing system also was performed. using the home-made "electrochemical capacitance analyzer", concentration effect of the supporting electrolyte to the basis capacitance and the total capacitance change resulted front the combination of calmodulin with ca2+ were studied. the stability and re-productability of the sensor also were examined. the results showed that for the determination of ca2+, the linear range extended from 10(-8) mol/l, to 3 10(-6) mol/l and the detection limit was 1 10(-9) mol/l. the sensor would kept its stability for one month by immersed in 0.02 mol/l (ph 7.0) pbs and stored at 4 c. the sensor also could be re-produced by edta and the basis capacitance varied less than 0.3% after reproduction. this work provides a new niethod for the determination of ca2+. |
| WOS关键词 | MOLECULAR RECOGNITION ; CALMODULIN ; SUBSTRATE ; MEMBRANE ; CELL |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000245482900008 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382176 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang Xiu-Rong |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi Bin,Yang Xiu-Rong. A new type of ca2+ sensor based on direct electrochemical capacitance method[J]. Chinese journal of analytical chemistry,2007,35(2):196-200. |
| APA | Qi Bin,&Yang Xiu-Rong.(2007).A new type of ca2+ sensor based on direct electrochemical capacitance method.Chinese journal of analytical chemistry,35(2),196-200. |
| MLA | Qi Bin,et al."A new type of ca2+ sensor based on direct electrochemical capacitance method".Chinese journal of analytical chemistry 35.2(2007):196-200. |
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来源：中国科学院大学
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