Hepatitis b virus core antigen epitopes presented by hla-a2 single-chain trimers induce functional epitope-specific cd8(+) t-cell responses in hla-a2.1/kb transgenic mice
文献类型：期刊论文
| 作者 | Zhang, Yuxia; Li, Shu; Shan, Ming; Pan, Xuwen; Zhuang, Ke; He, Lihua; Gould, Keith; Tien, Po |
| 刊名 | Immunology
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 121期号:1页码:105-112 |
| 关键词 | Cytotoxic t lymphocytes Hepatitis b virus Human leucocyte antigen a2 single-chain trimer Human leucocyte antigen a2 transgenic mice |
| ISSN号 | 0019-2805 |
| DOI | 10.1111/j.1365-2567.2007.02543.x |
| 通讯作者 | Tien, po(tienpo@sun.im.ac.cn) |
| 英文摘要 | The potency of cd8(+) cytotoxic t lymphocyte (ctl) responses toward core antigen has been shown to affect the outcomes of hepatitis b virus (hbv) infection. since single-chain trimers (sct) composed of peptide epitope beta(2)-microglobulin (beta(2)m) and major histocompatiblity complex (mhc) class i heavy chain covalently linked together in a single molecule have been shown to stimulate efficient ctl responses, we investigated the properties of human leucocyte antigen (hla)-a2 scts encoding the hbv core antigen (hbcag) epitopes c18-27 and c107-115. transfection of nih-3t3 cells with pcdna3.0-sct-c18-27 and sct-c107-115 leads to stable presentation of hbcag epitopes at the cell surface. hla-a2.1/kb transgenic mice vaccinated with the sct constructs, either as a dna vaccine alone or followed by a boost with recombinant vaccinia virus, were shown to generate hbcag-specific ctl responses by enzyme-linked immunospot assay (elispot) and in vitro interferon-gamma release experiments. hbcag-specific ctls from vaccinated hla-a2.1/kb transgenic mice were able to inhibit hbv surface and e antigen expression as indicated by hepg2.2.15 cells. our data indicate that a dna vaccine encoding a human hla-a2 sct with hbv epitopes can lead to stable, enhanced hbv core antigen presentation, and may be useful for the control of hbv infection in hla-a2-positive hbv carriers. |
| WOS关键词 | DNA VACCINE ; CTL ; LYMPHOCYTES ; PROTEIN ; IMMUNIZATION ; SURFACE ; LIVER ; TUBERCULOSIS ; IMMUNITY ; PEPTIDE |
| WOS研究方向 | Immunology |
| WOS类目 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000245399800010 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382271 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tien, Po |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Ctr Mol Virol, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 4.Univ London Imperial Coll Sci Technol & Med, London SW7 2AY, England |
| 推荐引用方式 GB/T 7714 | Zhang, Yuxia,Li, Shu,Shan, Ming,et al. Hepatitis b virus core antigen epitopes presented by hla-a2 single-chain trimers induce functional epitope-specific cd8(+) t-cell responses in hla-a2.1/kb transgenic mice[J]. Immunology,2007,121(1):105-112. |
| APA | Zhang, Yuxia.,Li, Shu.,Shan, Ming.,Pan, Xuwen.,Zhuang, Ke.,...&Tien, Po.(2007).Hepatitis b virus core antigen epitopes presented by hla-a2 single-chain trimers induce functional epitope-specific cd8(+) t-cell responses in hla-a2.1/kb transgenic mice.Immunology,121(1),105-112. |
| MLA | Zhang, Yuxia,et al."Hepatitis b virus core antigen epitopes presented by hla-a2 single-chain trimers induce functional epitope-specific cd8(+) t-cell responses in hla-a2.1/kb transgenic mice".Immunology 121.1(2007):105-112. |
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来源：中国科学院大学
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