Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography
文献类型：期刊论文
| 作者 | Zhang, Yu; Chen, Juan; Zhao, Liang; Shi, Yan-Ping |
| 刊名 | Biomedical chromatography
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 21期号:9页码:987-992 |
| 关键词 | Red clover Micellar electrokinetic capillary chromatography Daidzein Formononetin Genistein Biochanin a |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.843 |
| 通讯作者 | Shi, yan-ping(shiyp@lzb.ac.cn) |
| 英文摘要 | A micellar electrokinetic capillary chromatography (mecc) method has been developed for the determination of the four isoflavones, i.e. biochanin a, formononetin, genstein and daidzein in red clover (trifolium pratense l). the effect of running buffer ph and concentration were investigated. an electrolyte composed of 30 mm borate, 20 mm sodium dodecyl sulfate (sds) and 4 mg/ml hp-beta-cd containing 5% (v/v) ethanol at ph 10.1 provides a satisfactory separation for all the analytes. the applied voltage was 25 kv, and the capillary temperature was kept constant at 25 degrees c with a uv detection at 254 run. the relative standard deviations (rsd) of the migration time and peak area were less than 1.73 and 3.94% (intra-day), and 2.29 and 4.38% (inter-day), respectively, under the optimized separation conditions. regression equations revealed a good linear relationship between the peak area of each compound and its concentration. the contents of the four compounds in red clover were successfully determined with satisfactory repeatability and recovery. copyright (c) 2007 john wiley & sons, ltd. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRIC DETECTION ; TRIFOLIUM-PRATENSE ; REPLACEMENT THERAPY ; EXTRACTS ; ELECTROPHORESIS ; QUANTIFICATION ; ULTRAVIOLET ; FLAVONOIDS ; ESTROGEN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000248829700013 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382309 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shi, Yan-Ping |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Chen, Juan,Zhao, Liang,et al. Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography[J]. Biomedical chromatography,2007,21(9):987-992. |
| APA | Zhang, Yu,Chen, Juan,Zhao, Liang,&Shi, Yan-Ping.(2007).Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography.Biomedical chromatography,21(9),987-992. |
| MLA | Zhang, Yu,et al."Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography".Biomedical chromatography 21.9(2007):987-992. |
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来源：中国科学院大学
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