Crystal structure of homoserine o-acetyltransferase from leptospira interrogans
文献类型：期刊论文
| 作者 | Wang, Mingzhu; Liu, Lin; Wang, Yanli; Wei, Zhi-Yi; Zhang, Ping; Li, Ylkun; Jiang, Xiaohua; Xu, Hang; Gong, Welmin |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2007-11-30 |
| 卷号 | 363期号:4页码:1050-1056 |
| 关键词 | Homoserine o-acetyltransferase Crystal structure Alpha/beta hydrolase superfamily Catalytic triad Double conformations |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.08.153 |
| 通讯作者 | Xu, hang(hxu@moon.ibp.ac.cn) |
| 英文摘要 | Homoserine o-acetyltransferase (hta, ec 2.3.1.31) initiates methionine biosynthesis pathway by catalyzing the transfer of acetyl group from acetyl-coa to homoserine. this study reports the crystal structure of hta from leptospira interrogans determined at 2.2a resolution using selenomethionyl single-wavelength anomalous diffraction method. hta is modular and consists of two structurally distinct domains-a core alpha/beta domain containing the catalytic site and a helical bundle called the lid domain. overall, the structure fold belongs to alpha/beta hydrolase superfamily with the characteristic 'catalytic triad' residues in the active site. detailed structure analysis showed that the catalytic histidine and serine are both present in two conformations, which may be involved in the catalytic mechanism for acetyl transfer. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | SULFUR AMINO-ACIDS ; SACCHAROMYCES-CEREVISIAE ; METHIONINE BIOSYNTHESIS ; CRYSTALLIZATION ; TRANSACETYLASE ; MECHANISM ; REVEALS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000250456400029 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382352 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, Hang |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ Sci & Technol China, USTC Joint Lab Prot Sci, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Mingzhu,Liu, Lin,Wang, Yanli,et al. Crystal structure of homoserine o-acetyltransferase from leptospira interrogans[J]. Biochemical and biophysical research communications,2007,363(4):1050-1056. |
| APA | Wang, Mingzhu.,Liu, Lin.,Wang, Yanli.,Wei, Zhi-Yi.,Zhang, Ping.,...&Gong, Welmin.(2007).Crystal structure of homoserine o-acetyltransferase from leptospira interrogans.Biochemical and biophysical research communications,363(4),1050-1056. |
| MLA | Wang, Mingzhu,et al."Crystal structure of homoserine o-acetyltransferase from leptospira interrogans".Biochemical and biophysical research communications 363.4(2007):1050-1056. |
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来源：中国科学院大学
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