Expression and antibody preparation of pou transcription factor qbrn-1
文献类型：期刊论文
| 作者 | Lan, Lei; Liu, Mingnan; Liu, Ying; He, Rongqiao |
| 刊名 | Protein and peptide letters
 |
| 出版日期 | 2007 |
| 卷号 | 14期号:1页码:7-11 |
| 关键词 | Antibody Development Kidney Nervous system Pou Qbrn-1 Quail |
| ISSN号 | 0929-8665 |
| 通讯作者 | He, rongqiao(herq@sun5.ibp.ac.cn) |
| 英文摘要 | The transcription factor brn-1, which belongs to pou-domain family, has been shown to play critical roles in the development of the nervous system. a cdna clone coding for a quail brn-1 homologue, qbrn-1, was isolated. to investigate whether this gene plays a role in the development of the quail nervous system, an anti-n-terminal peptide antibody was prepared. the coding region for amino acids 1-79 (the n-terminal domain of qbrn-1) was subcloned into trx fusion expression vector pet32c and introduced into the escherichia coli origami(de3) cells for efficient expression. after purification, trx-fused polypeptides, called trx-qbm-in, were used to immunize the rabbits to prepare polyclonal antibodies against qbrn-1. the produced and purified antiserum showed specificity not only to the in vitro expressed qbrn-1, but also to the natural qbrn-1 in tissues. immunolabeling on sections by the anti-qbrn-1 serum showed that qbrn-1 was specifically expressed in the developing spinal cord and kidney. this suggests that qbrn-1 may play some roles in the development of avian nervous system and kidney, and the preparation of anti-qbrn-1 polyclonal antibody will facilitate further detection of, and functional study on, qbrn-1 both in vivo and in vitro. |
| WOS关键词 | NERVOUS-SYSTEM ; DOMAIN FACTORS ; CRUCIAL ROLES ; FAMILY ; KIDNEY |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000245376700002 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382361 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, Rongqiao |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lan, Lei,Liu, Mingnan,Liu, Ying,et al. Expression and antibody preparation of pou transcription factor qbrn-1[J]. Protein and peptide letters,2007,14(1):7-11. |
| APA | Lan, Lei,Liu, Mingnan,Liu, Ying,&He, Rongqiao.(2007).Expression and antibody preparation of pou transcription factor qbrn-1.Protein and peptide letters,14(1),7-11. |
| MLA | Lan, Lei,et al."Expression and antibody preparation of pou transcription factor qbrn-1".Protein and peptide letters 14.1(2007):7-11. |
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来源：中国科学院大学
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