Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping
文献类型：期刊论文
| 作者 | Yang, Qingkai; Liang, Chunyang; Zhuang, Wen; Li, Jun; Deng, Huabing; Deng, Qiyun; Wang, Bin |
| 刊名 | Planta
 |
| 出版日期 | 2007 |
| 卷号 | 225期号:2页码:321-330 |
| 关键词 | Physical mapping Rice Thermo-sensitive genic male-sterile rice Tms5 gene |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-006-0353-6 |
| 通讯作者 | Wang, bin(bwang@genetics.ac.cn) |
| 英文摘要 | Previous research has demonstrated that the thermo-sensitive genic male-sterile (tgms) gene in rice was regulated by temperature. tgms rice is important to hybrid rice production because the application of the tgms system in two-line breeding is cost-effective, simple, efficient and overcomes the limitations of the cytoplasmic male sterility (cms) system. annongs is the first discovered and deeply studied tgms rice line in china. previous studies have suggested that annongs-1 and y58s, two derivative tgms lines of annongs, were both controlled by a single recessive gene named tms5, which was genetically mapped on chromosome 2. in the current study, three populations (annongs-1 x nanjing11, y58s x q611, and y58s x guanghui122) were developed to investigate the tms5 gene molecular map. analysis of recombination events of sterile samples, utilizing 125 probes covering the tms5 region, suggested that the tms5 gene was physically mapped to a 19 kb dna fragment between two markers, 4039-1 and 4039-2, located on the bac clone ap004039. following the construction of a physical map between the two markers, onac023, a member of the nac (nam-ataf-cuc-related) gene family, was identified as the candidate of the tms5 gene. |
| WOS关键词 | ORYZA-SATIVA-L. ; ASYMMETRIC INTERLACED PCR ; NO-APICAL-MERISTEM ; ARABIDOPSIS-THALIANA ; PATTERN-FORMATION ; BOUNDARIES ; CLONING ; DNA |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000242855000006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382430 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Bin |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Hunan Hybrid Rice Res Ctr, Changsha 410125, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Qingkai,Liang, Chunyang,Zhuang, Wen,et al. Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping[J]. Planta,2007,225(2):321-330. |
| APA | Yang, Qingkai.,Liang, Chunyang.,Zhuang, Wen.,Li, Jun.,Deng, Huabing.,...&Wang, Bin.(2007).Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping.Planta,225(2),321-330. |
| MLA | Yang, Qingkai,et al."Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping".Planta 225.2(2007):321-330. |
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来源：中国科学院大学
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