中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'

文献类型:期刊论文

作者Wang, Xiaojun; Jin, Liang; Li, Mei; Mao, Minan; Zhao, Haiqing; Xu, Yaozu
刊名Journal of horticultural science & biotechnology
出版日期2007-09-01
卷号82期号:5页码:781-785
ISSN号1462-0316
通讯作者Xu, yaozu(mrxu0106@163.com)
英文摘要An efficient protocol is outlined for the establishment of proliferating cell cluster cultures using a bioreactor, and subsequent regeneration of the aromatic plant, lavandula angustifolia 'munstead'. red calli were obtained by culturing leaflet explants on basal ms medium supplemented with 0.23 mu m 2,4-dichlorophenoxyacetic (2,4-d) and 2.22 mu m 6-benzylaminopurine (ba), and subsequent sub-culturing. calli were then transferred to basal ms liquid medium containing 0.23 mu m 2,4-d and 2.22 mu m ba to initiate suspension cultures. cell clusters were obtained by sieving the initial suspensions and sub-culturing them in basal ms liquid medium supplemented with 0.23 mu m 2,4-d plus 2.22 mu m ba and 1.89 mu m abscisic acid, by means of shake culture, or bioreactor culture. after 20 d of culture, cell clusters were transferred to basal ms medium containing 1.14 mu m indole-3-acetic acid and produced a high number of regenerated plants, which were successfully transferred to soil.
WOS关键词ROSMARINIC ACID PRODUCTION ; EMERIC EX LOISELEUR ; MEDIATED TRANSFORMATION ; SUSPENSION-CULTURES ; VERA ; CALLUS ; GROWTH ; MICROPROPAGATION ; ACCUMULATION ; LATIFOLIA
WOS研究方向Agriculture
WOS类目Horticulture
语种英语
WOS记录号WOS:000250489300017
出版者HEADLEY BROTHERS LTD
URI标识http://www.irgrid.ac.cn/handle/1471x/2382465
专题中国科学院大学
通讯作者Xu, Yaozu
作者单位1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100830, Peoples R China
3.Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xiaojun,Jin, Liang,Li, Mei,et al. Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'[J]. Journal of horticultural science & biotechnology,2007,82(5):781-785.
APA Wang, Xiaojun,Jin, Liang,Li, Mei,Mao, Minan,Zhao, Haiqing,&Xu, Yaozu.(2007).Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'.Journal of horticultural science & biotechnology,82(5),781-785.
MLA Wang, Xiaojun,et al."Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'".Journal of horticultural science & biotechnology 82.5(2007):781-785.

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来源:中国科学院大学

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