Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'
文献类型:期刊论文
作者 | Wang, Xiaojun; Jin, Liang; Li, Mei; Mao, Minan; Zhao, Haiqing; Xu, Yaozu |
刊名 | Journal of horticultural science & biotechnology
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出版日期 | 2007-09-01 |
卷号 | 82期号:5页码:781-785 |
ISSN号 | 1462-0316 |
通讯作者 | Xu, yaozu(mrxu0106@163.com) |
英文摘要 | An efficient protocol is outlined for the establishment of proliferating cell cluster cultures using a bioreactor, and subsequent regeneration of the aromatic plant, lavandula angustifolia 'munstead'. red calli were obtained by culturing leaflet explants on basal ms medium supplemented with 0.23 mu m 2,4-dichlorophenoxyacetic (2,4-d) and 2.22 mu m 6-benzylaminopurine (ba), and subsequent sub-culturing. calli were then transferred to basal ms liquid medium containing 0.23 mu m 2,4-d and 2.22 mu m ba to initiate suspension cultures. cell clusters were obtained by sieving the initial suspensions and sub-culturing them in basal ms liquid medium supplemented with 0.23 mu m 2,4-d plus 2.22 mu m ba and 1.89 mu m abscisic acid, by means of shake culture, or bioreactor culture. after 20 d of culture, cell clusters were transferred to basal ms medium containing 1.14 mu m indole-3-acetic acid and produced a high number of regenerated plants, which were successfully transferred to soil. |
WOS关键词 | ROSMARINIC ACID PRODUCTION ; EMERIC EX LOISELEUR ; MEDIATED TRANSFORMATION ; SUSPENSION-CULTURES ; VERA ; CALLUS ; GROWTH ; MICROPROPAGATION ; ACCUMULATION ; LATIFOLIA |
WOS研究方向 | Agriculture |
WOS类目 | Horticulture |
语种 | 英语 |
WOS记录号 | WOS:000250489300017 |
出版者 | HEADLEY BROTHERS LTD |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382465 |
专题 | 中国科学院大学 |
通讯作者 | Xu, Yaozu |
作者单位 | 1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100830, Peoples R China 3.Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xiaojun,Jin, Liang,Li, Mei,et al. Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'[J]. Journal of horticultural science & biotechnology,2007,82(5):781-785. |
APA | Wang, Xiaojun,Jin, Liang,Li, Mei,Mao, Minan,Zhao, Haiqing,&Xu, Yaozu.(2007).Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'.Journal of horticultural science & biotechnology,82(5),781-785. |
MLA | Wang, Xiaojun,et al."Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, lavandula angustifolia 'munstead'".Journal of horticultural science & biotechnology 82.5(2007):781-785. |
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来源:中国科学院大学
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