Simply and reliably integrating micro heaters/sensors in a monolithic PCR-CE microfluidic genetic analysis system
文献类型：期刊论文
| 作者 | Zhong, Runtao1,2; Pan, Xiaoyan1,2; Jiang, Lei1; Dai, Zhongpeng1; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 30期号:8页码:1297-1305 |
| 关键词 | Genetic analysis Integrated microsystem Microfabricated heater PCR-CE microchip Sensor |
| 产权排序 | 1;1 |
| 通讯作者 | 秦建华 ; 林炳承 |
| 英文摘要 | a novel fabrication process was presented to construct a monolithic integrated pcr-ce microfluidic dna analysis system as a step toward building a total genetic analysis microsystem. microfabricated titanium/platinum (ti/pt) heaters and resistance temperature detectors (rtds) were integrated on the backside of a bonded glass chip to provide good thermal transfer and precise temperature detection for the drilled pcr-wells. this heater/rtd integration procedure was simple and reliable, and the resulting metal layer can be easily renewed when the ti/pt layer was damaged in later use or novel heater/rtd design was desired. a straightforward "rtd-calibration" method was employed to optimize the chip-based thermal cycling conditions. this method was convenient and rapid, comparing with a conventional rtd-calibration/temperature adjustment method. the highest ramping rates of 14 degrees c/s for heating and 5 degrees c/s for cooling in a 3-mu l reaction volume allow 30 complete pcr cycles in about 33 min. after effectively passivating the pcr-well surface, successful lambda-phage dna amplifications were achieved using a two- or three-temperature cycling protocol. the functionality and performance of the integrated microsystem were demonstrated by successful amplification and subsequent on-line separation/sizing of lambda-phage dna. a rapid assay for hepatitis b virus, one of the major human pathogens, was performed in less than 45 min, demonstrating that the developed pcr-ce microsystem was capable of performing automatic and high-speed genetic analysis. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | polymerase-chain-reaction ; capillary electrophoresis microsystem ; dna analysis ; amplification ; chip ; device ; separation ; microdevice ; extraction ; samples |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000265968300005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102057]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Runtao,Pan, Xiaoyan,Jiang, Lei,et al. Simply and reliably integrating micro heaters/sensors in a monolithic PCR-CE microfluidic genetic analysis system[J]. electrophoresis,2009,30(8):1297-1305. |
| APA | Zhong, Runtao,Pan, Xiaoyan,Jiang, Lei,Dai, Zhongpeng,Qin, Jianhua,&Lin, Bingcheng.(2009).Simply and reliably integrating micro heaters/sensors in a monolithic PCR-CE microfluidic genetic analysis system.electrophoresis,30(8),1297-1305. |
| MLA | Zhong, Runtao,et al."Simply and reliably integrating micro heaters/sensors in a monolithic PCR-CE microfluidic genetic analysis system".electrophoresis 30.8(2009):1297-1305. |
入库方式： OAI收割
来源：大连化学物理研究所
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