Microvalves actuated sandwich immunoassay on an integrated microfluidic system
文献类型：期刊论文
| 作者 | Gao, Xinghua1; Jiang, Lei1; Su, Xiaoou2; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 30期号:14页码:2481-2487 |
| 关键词 | Human immunoglobulin G Immunoassay Integration Microfluidics Microvalve |
| 产权排序 | 1;1 |
| 通讯作者 | 秦建华 ; 林炳承 |
| 英文摘要 | in this study, we developed an integrated and automatic microfluidic immunoassay system, which comprised a multi-layer immunoassay microfluidic chip and a rotary confocal lif scanner. the normally closed elastomeric microvalves were integrated on the multi-layer microfluidic chip to realize flexible reagents delivery for the rapid immunoassay. the various operations of immunoassay including coating, blocking and washing could be performed on a single chip with reduced reagents consumption and analysis time. the homemade rotary lif scanner was able to detect the signals from the multichannels sequentially in a short period of time, enabling the potential capability for high-throughput immunoassay. the performance of this system was demonstrated by the sandwich immunoassay of human immunoglobulin g with a lod < 10 ng/ml (s/n = 3). the linear range was from 500 ng/ml to 100 mu g/ml. this system has also been applied to the measurement of human immunoglobulin g from 15 real samples, demonstrating the potential of this platform for biomedical applications. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | large-scale integration ; on-chip ; valves ; samples ; device ; pumps ; assay |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000269041500010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102069]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agriprod, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xinghua,Jiang, Lei,Su, Xiaoou,et al. Microvalves actuated sandwich immunoassay on an integrated microfluidic system[J]. electrophoresis,2009,30(14):2481-2487. |
| APA | Gao, Xinghua,Jiang, Lei,Su, Xiaoou,Qin, Jianhua,&Lin, Bingcheng.(2009).Microvalves actuated sandwich immunoassay on an integrated microfluidic system.electrophoresis,30(14),2481-2487. |
| MLA | Gao, Xinghua,et al."Microvalves actuated sandwich immunoassay on an integrated microfluidic system".electrophoresis 30.14(2009):2481-2487. |
入库方式： OAI收割
来源：大连化学物理研究所
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