Molecular and functional analysis of phosphomannomutase (pmm) from higher plants and genetic evidence for the involvement of pmm in ascorbic acid biosynthesis in arabidopsis and nicotiana benthamiana
文献类型：期刊论文
| 作者 | Qian, Weiqiang; Yu, Chunmei; Qin, Huanju; Liu, Xin; Zhang, Aimin; Johansen, Ida Elisabeth; Wang, Daowen |
| 刊名 | Plant journal
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 49期号:3页码:399-413 |
| 关键词 | Phosphomannomutase Arabidopsis Ascorbic acid Yeast complementation Recombinant protein Virus induced gene silencing |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/j.1365-313x.2006.02967.x |
| 通讯作者 | Wang, daowen(dwwang@genetics.ac.cn) |
| 英文摘要 | Phosphomannomutase (pmm) catalyzes the interconversion of mannose-6-phosphate and mannose-1-phosphate. however, systematic molecular and functional investigations on pmm from higher plants have hitherto not been reported. in this work, pmm cdnas were isolated from arabidopsis, nicotiana benthamiana, soybean, tomato, rice and wheat. amino acid sequence comparisons indicated that plant pmm proteins exhibited significant identity to their fungal and mammalian orthologs. in line with the similarity in primary structure, plant pmm complemented the sec53-6 temperature sensitive mutant of saccharomyces cerevisiae. histidine-tagged arabidopsis pmm (atpmm) purified from escherichia coli converted mannose-1-phosphate into mannose-6-phosphate and glucose-1-phosphate into glucose-6-phosphate, with the former reaction being more efficient than the latter one. in arabidopsis and n. benthamiana, pmm was constitutively expressed in both vegetative and reproductive organs. reducing the pmm expression level through virus-induced gene silencing caused a substantial decrease in ascorbic acid (asa) content in n. benthamiana leaves. conversely, raising the pmm expression level in n. benthamiana using viral-vector-mediated ectopic expression led to a 20-50% increase in asa content. consistent with this finding, transgenic expression of an atpmm-gfp fusion protein in arabidopsis also increased asa content by 25-33%. collectively, this study improves our understanding on the molecular and functional properties of plant pmm and provides genetic evidence on the involvement of pmm in the biosynthesis of asa in arabidopsis and n. benthamiana plants. |
| WOS关键词 | L-GALACTOSE DEHYDROGENASE ; VITAMIN-C ; GDP-MANNOSE ; SACCHAROMYCES-CEREVISIAE ; PROTEIN GLYCOSYLATION ; SEC53 GENE ; YEAST ; THALIANA ; MUTANTS ; EXPRESSION |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000243637900003 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382890 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Daowen |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Shijingshan, Peoples R China 3.Danish Inst Agr Sci, Dept Genet & Biotechnol, DK-1871 Frederiksberg, Denmark |
| 推荐引用方式 GB/T 7714 | Qian, Weiqiang,Yu, Chunmei,Qin, Huanju,et al. Molecular and functional analysis of phosphomannomutase (pmm) from higher plants and genetic evidence for the involvement of pmm in ascorbic acid biosynthesis in arabidopsis and nicotiana benthamiana[J]. Plant journal,2007,49(3):399-413. |
| APA | Qian, Weiqiang.,Yu, Chunmei.,Qin, Huanju.,Liu, Xin.,Zhang, Aimin.,...&Wang, Daowen.(2007).Molecular and functional analysis of phosphomannomutase (pmm) from higher plants and genetic evidence for the involvement of pmm in ascorbic acid biosynthesis in arabidopsis and nicotiana benthamiana.Plant journal,49(3),399-413. |
| MLA | Qian, Weiqiang,et al."Molecular and functional analysis of phosphomannomutase (pmm) from higher plants and genetic evidence for the involvement of pmm in ascorbic acid biosynthesis in arabidopsis and nicotiana benthamiana".Plant journal 49.3(2007):399-413. |
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来源：中国科学院大学
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