Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.)
文献类型：期刊论文
| 作者 | Wu, Suowei; Yu, Zhanwang; Wang, Fengge; Li, Weihua; Ye, Chunjiang; Li, Jun; Tang, Jihua; Ding, Junqiang; Zhao, Jiuran; Wang, Bin |
| 刊名 | Molecular biotechnology
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 36期号:2页码:102-112 |
| 关键词 | Maize (zea mays l.) phosphoethanolamine n-methyltransferase promoter Tail-pcr Transgenic plants |
| ISSN号 | 1073-6085 |
| DOI | 10.1007/s12033-007-0009-1 |
| 通讯作者 | Wang, bin(bwang@genetics.ac.cn) |
| 英文摘要 | N-methylation of phosphoethanolamine, the committing step in choline (cho) biosynthesis in plants, is catalyzed by s-adenosyl-l-methionine: phosphoethanolamine n-methyltransferase (peamt, ec 2.1.1.103). herein we report the cloning and characterization of the novel maize phosphoethanolarnine n-methyltransferase gene (zmpeamt1) using a combination of bioinformatics and a pcr-based allele mining strategy. the cdna sequence of zmpeamt] gene is 1,806 bp in length and translates a 495 amino acids peptide. the upstream promoter sequence of zmpeamt1 were obtained by tail-pcr, and contained four kinds of putative cis-acting regulatory elements, including stress-responsive elements, phytohormone-responsive elements, pollen developmental special activation elements, and light-induced signal transduction elements, as well as several other structural features in common with the promoter of rice and arabidopsis homologies. rt-pcr analysis showed that expression of zmpeamt1 was induced by salt stress and suppressed by high temperature. over-expression of zmpeamt1 enhanced the salt tolerance, root length, and silique number in transgenic arabidopsis. these data indicated that zmpeamt1 maybe involved in maize root development and stress resistance, and maybe having a potential application in maize genetic engineering. |
| WOS关键词 | TRANSGENIC TOBACCO PLANTS ; CIS-REGULATORY ELEMENTS ; ARABIDOPSIS-THALIANA ; GLYCINE BETAINE ; TRANSCRIPTION FACTORS ; PHOSPHATIDIC-ACID ; CHOLINE OXIDASE ; CODA GENE ; GCC-BOX ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000247271300003 |
| 出版者 | HUMANA PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382904 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Bin |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing 100089, Peoples R China 4.Henan Agr Univ, Coll Agron, Zhengzhou 450002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Suowei,Yu, Zhanwang,Wang, Fengge,et al. Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.)[J]. Molecular biotechnology,2007,36(2):102-112. |
| APA | Wu, Suowei.,Yu, Zhanwang.,Wang, Fengge.,Li, Weihua.,Ye, Chunjiang.,...&Wang, Bin.(2007).Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.).Molecular biotechnology,36(2),102-112. |
| MLA | Wu, Suowei,et al."Cloning, characterization, and transformation of the phosphoethanolamine n-methyltransferase gene (zmpeamt1) in maize (zea mays l.)".Molecular biotechnology 36.2(2007):102-112. |
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来源：中国科学院大学
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