Molecular cloning and expression analysis of a monosaccharide transporter gene osmst4 from rice (oryza sativa l.)
文献类型：期刊论文
| 作者 | Wang, Yongqin; Xu, Honglin; Wei, Xiaoli; Chai, Chenglin; Xiao, Yuguo; Zhang, Yu; Chen, Bin; Xiao, Guifang; Ouwerkerk, Pieter B. F.; Wang, Mei |
| 刊名 | Plant molecular biology
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 65期号:4页码:439-451 |
| 关键词 | Cdna cloning Gene expression Monosaccharide transporter Oryza sativa l Seed development |
| ISSN号 | 0167-4412 |
| DOI | 10.1007/s11103-007-9228-x |
| 通讯作者 | Zhu, zhen(zzhu@genetics.ac.cn) |
| 英文摘要 | Monosaccharide transporters mediate the membrane transport of a variable range of monosaccharides, which plays a crucial role in sugar distribution throughout the plant. to investigate the significance of monosaccharide transporters for rice (oryza sativa l.) seed development, cdna of a new putative monosaccharide transporter gene osmst4 was isolated. the deduced osmst4 protein shows typical features of monosaccharide transporters, and shares high homology with other plant homologues. heterologous expression in yeast (saccharomyces cerevisiae) showed that osmst4 is a functional monosaccharide transporter capable of transporting glucose, fructose, mannose and galactose. transcriptional analysis revealed that osmst4 is expressed in all tested organs/tissues. in developing caryopses, its expression is high at the early and middle grain filling stages, and declines gradually to low levels after that. further analysis revealed that it is expressed in both the maternal tissue and the filial tissue, with its highest expression in embryo. cellular location in young caryopses through rna in situ hybridization showed that osmst4 mrna mainly accumulates in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm. the expression pattern of osmst4 was further confirmed by histochemical analysis of the osmst4-promoter-beta-glucuronidase (gus) transgenic rice plants. these data indicate that osmst4 is actively involved in monosaccharides supply for seed development during the course of grain filling. in addition, the cell type-specific expression patterns of osmst4 in other sink and source tissues were also investigated, and its corresponding physiological roles were discussed. |
| WOS关键词 | CELL-WALL INVERTASE ; PLASTIDIC GLUCOSE TRANSLOCATOR ; SUCROSE TRANSPORTER ; SUGAR TRANSPORTERS ; HEXOSE TRANSPORTERS ; SEED DEVELOPMENT ; HIGHER-PLANTS ; CDNA CLONING ; ARABIDOPSIS ; FAMILY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000250208200007 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382917 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Zhen |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Leiden Univ, Inst Biol, Dept Mol & Dev Genet, NL-2300 RA Leiden, Netherlands 4.TNO Qual Life, NL-2333 CK Leiden, Netherlands |
| 推荐引用方式 GB/T 7714 | Wang, Yongqin,Xu, Honglin,Wei, Xiaoli,et al. Molecular cloning and expression analysis of a monosaccharide transporter gene osmst4 from rice (oryza sativa l.)[J]. Plant molecular biology,2007,65(4):439-451. |
| APA | Wang, Yongqin.,Xu, Honglin.,Wei, Xiaoli.,Chai, Chenglin.,Xiao, Yuguo.,...&Zhu, Zhen.(2007).Molecular cloning and expression analysis of a monosaccharide transporter gene osmst4 from rice (oryza sativa l.).Plant molecular biology,65(4),439-451. |
| MLA | Wang, Yongqin,et al."Molecular cloning and expression analysis of a monosaccharide transporter gene osmst4 from rice (oryza sativa l.)".Plant molecular biology 65.4(2007):439-451. |
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来源：中国科学院大学
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