Identification of necrophagous fly species using issr and scar markers
文献类型：期刊论文
| 作者 | He, Lin; Wang, Sibao; Miao, Xuexia; Wu, Hong; Huang, Yongping |
| 刊名 | Forensic science international
 |
| 出版日期 | 2007-05-24 |
| 卷号 | 168期号:2-3页码:148-153 |
| 关键词 | Forensic insects Necrophagous flies Molecular markers Issr Scar |
| ISSN号 | 0379-0738 |
| DOI | 10.1016/j.forsciint.2006.07.006 |
| 通讯作者 | Wu, hong(hongwu@sibs.ac.cn) |
| 英文摘要 | Necrophagous fly is the most common insect evidence collected during a death investigation. we analyzed the dna polymorphism among the five forensically important fly species, namely, lucilia sericata, aldrichina grahami, chrysomya megacephala, parasarcophaga crassipalpis and musca domestica using inter simple sequence repeat (issr) method. nine issr primers selected from 18 primers could amplify 105 clear and stable bands, of which 95 bands were polymorphic. some primers produced completely different band pattern in different species, indicating that they can be used to identify these species. aiming at obtaining more reliable markers that might be universally used, we started an effort to convert species-specific issr fragments into the sequence-characterized amplified region (scar) markers that can be used for the molecular diagnosis of the five species. (c) 2006 elsevier ireland ltd. all rights reserved. |
| WOS关键词 | GENETIC DIVERSITY ; DIPTERA ; BLOWFLIES ; RAPD ; CALLIPHORIDAE ; AUSTRALIA ; PLANTS |
| WOS研究方向 | Legal Medicine |
| WOS类目 | Medicine, Legal |
| 语种 | 英语 |
| WOS记录号 | WOS:000246729200011 |
| 出版者 | ELSEVIER IRELAND LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2382971 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wu, Hong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Lin,Wang, Sibao,Miao, Xuexia,et al. Identification of necrophagous fly species using issr and scar markers[J]. Forensic science international,2007,168(2-3):148-153. |
| APA | He, Lin,Wang, Sibao,Miao, Xuexia,Wu, Hong,&Huang, Yongping.(2007).Identification of necrophagous fly species using issr and scar markers.Forensic science international,168(2-3),148-153. |
| MLA | He, Lin,et al."Identification of necrophagous fly species using issr and scar markers".Forensic science international 168.2-3(2007):148-153. |
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来源：中国科学院大学
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