Interaction of cationic vesicle with ribonucleotides (amp, adp, and atp) and physicochemical characterization of dodab/ribonucleotides complexes
文献类型：期刊论文
| 作者 | Liu, Shuhua; Lu, Gongxuan |
| 刊名 | Biophysical chemistry
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 127期号:1-2页码:19-27 |
| 关键词 | Ribonucleotides (amp, adp, and atp) Dodab Transmission electron microscopy Gel exclusion and uv spectroscopy Zeta-potentials and electrophoretic mobilities Dynamic light scattering |
| ISSN号 | 0301-4622 |
| DOI | 10.1016/j.bpc.2006.11.007 |
| 通讯作者 | Lu, gongxuan(gxlu@lzb.ac.en) |
| 英文摘要 | The interaction between ribonucleotides (amp, adp, and atp) and cationic vesicles prepared from dioctadecyldimethylammonium bromide (dodab) were investigated in detail. the physicochemical properties of ribonucleotides/cationic lipid complexes were present. gel exclusion-uv spectroscopic results showed that all the charge ratios of dodab/ribonucleotides (amp, adp, and atp) are 2:1 when the maximal ribonucleotides were adsorbed onto dodab, while the molar ratios were different, e.g., 2:1 for dodab/amp, 4:1 for dodab/adp and 6:1 for dodab/atp. these differences may be attributed to the different anion charges of amp, adp and atp. the results demonstrated that ribonucleotides combined with dodab vesicles with the electrostatic attraction in the complexation of dodab and ribonucleotides. transmission electron microscopic results revealed the different extents of aggregation of cationic vesicles in the complexation process of ribonucleotides with cationic lipid. the variation dependence of zeta-potentials or electrophoretic mobilities on vesicle size was also different. the zeta-potentials and electrophoretic mobilities of the dodab vesicles (0.01 and 0.02 mm) gradually decreased when the ribonucleotide concentration increased. however, the mean diameters of the dodab vesicles (0.1 and 0.5 mm) gradually increased when the ribonucleotide concentration increased. (c) 2006 elsevier b.v. all rights reserved. |
| WOS关键词 | DIOCTADECYLDIMETHYLAMMONIUM BROMIDE ; CHLORIDE VESICLES ; L-ARGININE ; DNA ; LIPOSOMES ; TRANSFECTION ; AMPHIPHILE ; DELIVERY ; SIZE ; OLIGONUCLEOTIDES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Chemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical |
| 语种 | 英语 |
| WOS记录号 | WOS:000245168700003 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2383029 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lu, Gongxuan |
| 作者单位 | 1.Lanzhou Univ, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Shuhua,Lu, Gongxuan. Interaction of cationic vesicle with ribonucleotides (amp, adp, and atp) and physicochemical characterization of dodab/ribonucleotides complexes[J]. Biophysical chemistry,2007,127(1-2):19-27. |
| APA | Liu, Shuhua,&Lu, Gongxuan.(2007).Interaction of cationic vesicle with ribonucleotides (amp, adp, and atp) and physicochemical characterization of dodab/ribonucleotides complexes.Biophysical chemistry,127(1-2),19-27. |
| MLA | Liu, Shuhua,et al."Interaction of cationic vesicle with ribonucleotides (amp, adp, and atp) and physicochemical characterization of dodab/ribonucleotides complexes".Biophysical chemistry 127.1-2(2007):19-27. |
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来源：中国科学院大学
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