The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs
文献类型：期刊论文
| 作者 | Wang, Bin; Han, Jin; Gao, Yuan; Xiao, Zhifeng; Chen, Bing; Wang, Xia; Zhao, Wenxue; Dai, Jianwu |
| 刊名 | Neuroscience letters
 |
| 出版日期 | 2007-06-29 |
| 卷号 | 421期号:3页码:191-196 |
| 关键词 | Oecs Adscs Differentiation Co-culture Three-dimensional culture |
| ISSN号 | 0304-3940 |
| DOI | 10.1016/j.neulet.2007.04.081 |
| 通讯作者 | Dai, jianwu(jwdai@genetics.ac.cn) |
| 英文摘要 | Olfactory ensheathing cells (oecs) transplantation is a promising or potential therapy for spinal cord injury (sci). however, their clinical use is limited because of the availability. adipose-derived stem cells (adscs) have been identified as an alternative source of adult stein cells in recent years. adscs could be differentiated into various mesenchymal tissues cells such as chondrocytes, adipocytes, osteoblasts, and myocytes and also could be differentiated into neural lineages. in this study, we examined the feasibility of using adscs as a source of stem cells for the differentiation of oecs by co-culture approach. when co-cultured with oecs, the adscs on three-dimensional collagen scaffolds were differentiated into oec-like cells, with similar morphology and antigenic phenotypes (p75ntr+/nestin+/gfap-) of oecs. co-cultured adscs were positive for several important functional markers of mature oecs such as neurotrophic factor gdnf, bdnf and myelin protein plp and the conditioned medium of ofc-like cells could significantly promote drg neuron growth and axon sprouting without ngf supporting in contrast to that of the adscs. our results showed that adscs had the potential to differentiate into oec-like cells on the three-dimensional collagen scaffolds in vitro. (c) 2007 published by elsevier ireland ltd. |
| WOS关键词 | OLFACTORY ENSHEATHING CELLS ; SPINAL-CORD ; SCHWANN-CELLS ; IN-VITRO ; STROMAL CELLS ; ADULT CNS ; TRANSPLANTATION ; TISSUE ; REGENERATION ; EXPRESSION |
| WOS研究方向 | Neurosciences & Neurology |
| WOS类目 | Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000248152100002 |
| 出版者 | ELSEVIER IRELAND LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2383208 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dai, Jianwu |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Bin,Han, Jin,Gao, Yuan,et al. The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs[J]. Neuroscience letters,2007,421(3):191-196. |
| APA | Wang, Bin.,Han, Jin.,Gao, Yuan.,Xiao, Zhifeng.,Chen, Bing.,...&Dai, Jianwu.(2007).The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs.Neuroscience letters,421(3),191-196. |
| MLA | Wang, Bin,et al."The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs".Neuroscience letters 421.3(2007):191-196. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。