Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope
文献类型：期刊论文
| 作者 | Lv, Guojun1,2; Sun, Zhijie1,2; Li, Shuangyue1,2; Yu, Weiting1,2; Xie, Yubing1; Zhang, Ying1,2; Xie, Hongguo1,2; Li, Xiaoxia1,2; Wang, Wei1; Ma, Xiaojun1 |
| 刊名 | journal of biomedical materials research part a
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 90a期号:3页码:773-783 |
| 关键词 | cell encapsulation alginate membrane permeability protein fouling confocal laser scanning microscope (CLSM) |
| 产权排序 | 1;1 |
| 通讯作者 | 马小军 |
| 英文摘要 | microencapsulation of recombinant cells is a novel means for gene therapy. however, one of the major concerns is the relationship between the permeability of microcapsule and cell growth. many studies have focused on the permeability of empty microcapsule, but little is known about the effect of the cell growth on the permeability of a cell-contained microcapsule. a combination of fluorescence labeled protein and confocal laser scanning microscope (clsm) provides the information about the permeability changes during the cell growth. a decrease of membrane permeability was detected oil the 14th day. meanwhile, membrane surface protein fouling was also investigated. a significant increase of membrane surface protein content was detected on the 21st day. in order to study the effect of the permeability changes on the cell viability, the membrane of cell-contained microcapsules with different permeability was set up by incubating gel beads in poly-l-lysine for 5 and 30 min, respectively, to mimic the bovine serum albumin cutoff, and a retard of cell growth was found in 7 days' culture. these results showed that the protein fouling of the microcapsule membrane caused by the cell growth may be in important factor to influence cell viability. (c) 2008 wiley periodicals, inc. j biomed mater res 96a: 773-783, 2009 |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | engineering, biomedical ; materials science, biomaterials |
| 研究领域[WOS] | engineering ; materials science |
| 关键词[WOS] | microfiltration membranes ; alginate microcapsules ; poly(ethylene oxide) ; diffusion ; model ; microencapsulation ; macromolecules ; release ; gels ; transplantation |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000268860100017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102147]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Guojun,Sun, Zhijie,Li, Shuangyue,et al. Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope[J]. journal of biomedical materials research part a,2009,90a(3):773-783. |
| APA | Lv, Guojun.,Sun, Zhijie.,Li, Shuangyue.,Yu, Weiting.,Xie, Yubing.,...&Ma, Xiaojun.(2009).Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope.journal of biomedical materials research part a,90a(3),773-783. |
| MLA | Lv, Guojun,et al."Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope".journal of biomedical materials research part a 90a.3(2009):773-783. |
入库方式： OAI收割
来源：大连化学物理研究所
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