Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports
文献类型：期刊论文
| 作者 | Sun, J.1; Zhou, Y.2; Yuan, Z.3; Xu, G.1 |
| 刊名 | applied biochemistry and microbiology
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 45期号:5页码:478-483 |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | alcaligenes faecalis penicillin g acylase has several desired features over other penicillin g acylases and its use in industry requires immobilization. in this work, two novel supports zh-ep (epoxy type) and zh-ha (epoxy-amino type) were used to immobilize alcaligenes faecalis penicillin g acylase (afpga) with eupergit c as reference. the saturation of immobilized protein on zh-ep (269 mg/g, 116 h) and zh-ha (296 mg/g, 15 h) was obtained more rapidly than eupergit c (197 mg/g, 260 h). and the activity of immobilized afpga on zh-ep (520 u/g) and zh-ha (2200 u/g) was higher than that on eupergit c (310 u/g). the propertics of three immobilized enzymes were compared and no obvious difference was observed, which indicated that zh-ep and zh-ha were promised in industry. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology ; microbiology |
| 关键词[WOS] | multipoint covalent attachment ; salt-induced immobilization ; activated supports ; glutaryl acylase ; proteins ; performance ; sepabeads ; enzymes ; tool |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000269885800003 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102167]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, J.,Zhou, Y.,Yuan, Z.,et al. Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports[J]. applied biochemistry and microbiology,2009,45(5):478-483. |
| APA | Sun, J.,Zhou, Y.,Yuan, Z.,&Xu, G..(2009).Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports.applied biochemistry and microbiology,45(5),478-483. |
| MLA | Sun, J.,et al."Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports".applied biochemistry and microbiology 45.5(2009):478-483. |
入库方式： OAI收割
来源：大连化学物理研究所
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