中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports

文献类型:期刊论文

作者Sun, J.1; Zhou, Y.2; Yuan, Z.3; Xu, G.1
刊名applied biochemistry and microbiology
出版日期2009-09-01
卷号45期号:5页码:478-483
产权排序1;1
通讯作者许国旺
英文摘要alcaligenes faecalis penicillin g acylase has several desired features over other penicillin g acylases and its use in industry requires immobilization. in this work, two novel supports zh-ep (epoxy type) and zh-ha (epoxy-amino type) were used to immobilize alcaligenes faecalis penicillin g acylase (afpga) with eupergit c as reference. the saturation of immobilized protein on zh-ep (269 mg/g, 116 h) and zh-ha (296 mg/g, 15 h) was obtained more rapidly than eupergit c (197 mg/g, 260 h). and the activity of immobilized afpga on zh-ep (520 u/g) and zh-ha (2200 u/g) was higher than that on eupergit c (310 u/g). the propertics of three immobilized enzymes were compared and no obvious difference was observed, which indicated that zh-ep and zh-ha were promised in industry.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biotechnology & applied microbiology ; microbiology
研究领域[WOS]biotechnology & applied microbiology ; microbiology
关键词[WOS]multipoint covalent attachment ; salt-induced immobilization ; activated supports ; glutaryl acylase ; proteins ; performance ; sepabeads ; enzymes ; tool
收录类别SCI
原文出处false
语种英语
WOS记录号WOS:000269885800003
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/102167]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714
Sun, J.,Zhou, Y.,Yuan, Z.,et al. Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports[J]. applied biochemistry and microbiology,2009,45(5):478-483.
APA Sun, J.,Zhou, Y.,Yuan, Z.,&Xu, G..(2009).Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports.applied biochemistry and microbiology,45(5),478-483.
MLA Sun, J.,et al."Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports".applied biochemistry and microbiology 45.5(2009):478-483.

入库方式: OAI收割

来源:大连化学物理研究所

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