Columns switch recycling size exclusion chromatography for high resolution protein separation
文献类型：期刊论文
| 作者 | Yuan, Huiming1,2; Zhang, Lihua1; Zhang, Weibing3; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-10-16 |
| 卷号 | 1216期号:42页码:7024-7032 |
| 关键词 | Columns switch recycling size exclusion chromatography Multidimensional HPLC Protein separation |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | columns switch recycling size exclusion chromatography (csrsec) was proposed to achieve high resolution protein separation with good biocompatibility. proteins were firstly separated by two serially coupled sec columns, and fractions were in sequence switched back to the first column by two-position valves for further separation in terms of close-loop recycling until satisfactory resolution was achieved. compared to sec, the separation window was broadened by increasing column length via cycling without further increase on back pressure. compared to recycling sec (rsec), the overtaking of later eluted components by early eluted ones after several cycles could be avoided for complex sample analysis, by parking fractions in the second sec column before transferred in turn back to the first one for cycling ordinally. in our experiments, the baseline separation of five proteins with molecular weight ranging from 10 kda to 80 kda was achieved by csrsec. furthermore, a multidimensional csrsec-rplc platform was constructed, and peak capacity up to 3600 was obtained for protein separation. all these results demonstrated that csrsec is a promising protein separation mode with good biocompatibility, broadened separation window and improved resolution. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | capillary liquid-chromatography ; 2-dimensional hplc ; peak-capacity ; purification ; modulation ; phase ; lc |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000270750900007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102169]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.E China Univ Sci & Technol, Sch Chem & Mol Engn, Dept Chem, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Huiming,Zhang, Lihua,Zhang, Weibing,et al. Columns switch recycling size exclusion chromatography for high resolution protein separation[J]. journal of chromatography a,2009,1216(42):7024-7032. |
| APA | Yuan, Huiming,Zhang, Lihua,Zhang, Weibing,Liang, Zhen,&Zhang, Yukui.(2009).Columns switch recycling size exclusion chromatography for high resolution protein separation.journal of chromatography a,1216(42),7024-7032. |
| MLA | Yuan, Huiming,et al."Columns switch recycling size exclusion chromatography for high resolution protein separation".journal of chromatography a 1216.42(2009):7024-7032. |
入库方式： OAI收割
来源：大连化学物理研究所
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