Extraction and analysis of adenosine phosphate in cells of microcystis aeruginosa
文献类型：期刊论文
| 作者 | Dai Rui-Hua1,2; Liu Hui-Juan1; Qu Jiu-Hui1 |
| 刊名 | Chinese journal of analytical chemistry
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 35期号:12页码:1701-1705 |
| 关键词 | Microcystis aeruginosa Adenosine phosphate Extraction |
| ISSN号 | 0253-3820 |
| 通讯作者 | Qu jiu-hui(jhqu@rcees.ac.cn) |
| 英文摘要 | Four methods, perchloric acid extraction, organic solvent extraction, boiling bitter salt solution extraction and boiling bitter salt solution with ultrasonic extraction, were used to extract adenosine triphosphate atp), denosine diphosphate ( adp) and adensine monophosphate ( amp) from the cells of microcystis aeruginosa. bitter salt solution with ultrasonic extraction was testified to be the optimum method. the optimum volume of extracting solution was 2 ml by the method of boiling bitter salt solution with ultrasonic. when atp, adp and amp were put in boiling water bar, the concentration of atp and amp changed largely but the content of adp changed smaller. in this study, the cells were put into boiling water bar for 10 min and then were staved with ultrasonic for 10 min with bitter salt solution as extractive solution. this method was simple and nonpoisonous. a reversed phase high-performance liquid chromatography with uv detection was used for the determination of atp, adp and amp. they were distinctly separated within 10 min. moreover, the method has the advantage of high sensitivity, rapidity and accurcy. the recovery of atp, adp and amp was 88% -97%, 103% - 107%, 109% - 115% respectively. the relative standard deviations were less than 10%. |
| WOS关键词 | ADENYLATE ENERGY-CHARGE ; ENZYME ; LIVER |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000253934500003 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2383417 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qu Jiu-Hui |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Eco Environm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai Rui-Hua,Liu Hui-Juan,Qu Jiu-Hui. Extraction and analysis of adenosine phosphate in cells of microcystis aeruginosa[J]. Chinese journal of analytical chemistry,2007,35(12):1701-1705. |
| APA | Dai Rui-Hua,Liu Hui-Juan,&Qu Jiu-Hui.(2007).Extraction and analysis of adenosine phosphate in cells of microcystis aeruginosa.Chinese journal of analytical chemistry,35(12),1701-1705. |
| MLA | Dai Rui-Hua,et al."Extraction and analysis of adenosine phosphate in cells of microcystis aeruginosa".Chinese journal of analytical chemistry 35.12(2007):1701-1705. |
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来源：中国科学院大学
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