中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles

文献类型:期刊论文

作者Song, Cuihong1; Ye, Zhiqiang2; Wang, Guilan2; Jin, Dayong3; Yuan, Jingli1,2; Guan, Yafeng1; Piper, James3
刊名talanta
出版日期2009-06-30
卷号79期号:1页码:103-108
关键词Ruthenium complex Luminescent nanoparticles Bioimaging Time-gated luminescence Environmental pathogen
产权排序1;1
通讯作者袁景利 ; 关亚风
英文摘要luminescent ruthenium(ii) complex covalently bound silica nanoparticles have been prepared and used as a probe for time-gated luminescence bioimaging. the new nanoparticles were prepared by copolymerization of a luminescent ru(ii) complex tris(5-amino-1,10-phenanthroline)ruthenium(ii) conjugated with 3-aminopropyl(triethoxy)si lane (aps-ru conjugate), free (3 -aminopropyl)triethoxysilane (aps) and tetraethyl orthosilicate (teos) in a water-in-oil reverse microemulsion consisting of triton x-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia. characterization by transmission electron microscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, 64 +/- 4 nm in diameter. compared with the dye-doping nanoparticles, dye leakage of the new nanoparticles was remarkably decreased. in addition, it was found that the ru(ii) complex luminescence could be effectively enhanced with a longer luminescence lifetime (similar to 2.3 mu s) after forming the nanoparticles, which enables the nanoparticles to be suitable as a bioprobe for time-gated luminescence bioimaging applications. the nanoparticle-labeled streptavidin was prepared and successfully used for time-gated luminescence imaging detection of an environmental pathogen, giardia lamblia, with high specificity and sensitivity. (c) 2009 elsevier b.v. all rights reserved.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, analytical
研究领域[WOS]chemistry
关键词[WOS]giardia-lamblia ; bioconjugated nanoparticles ; fluorescent nanoparticles ; europium(iii) complex ; microscopy ; uv ; cryptosporidium ; excitation ; lifetimes ; bacteria
收录类别SCI
原文出处false
语种英语
WOS记录号WOS:000266187600018
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/102213]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dept Chem, Dalian 116012, Peoples R China
3.Macquarie Univ, MQ Photon Ctr, Fac Sci, Sydney, NSW 2109, Australia
推荐引用方式
GB/T 7714
Song, Cuihong,Ye, Zhiqiang,Wang, Guilan,et al. Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles[J]. talanta,2009,79(1):103-108.
APA Song, Cuihong.,Ye, Zhiqiang.,Wang, Guilan.,Jin, Dayong.,Yuan, Jingli.,...&Piper, James.(2009).Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles.talanta,79(1),103-108.
MLA Song, Cuihong,et al."Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles".talanta 79.1(2009):103-108.

入库方式: OAI收割

来源:大连化学物理研究所

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