Genetic modification of industrial yeast strains to obtain controllable newflo flocculation property and lower diacetyl production
文献类型：期刊论文
| 作者 | Wang, Dongli1,2; Wang, Zhaoyue1; Liu, Nan1; He, Xiuping1; Zhang, Borun1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 30期号:11页码:2013-2018 |
| 关键词 | Adh2 promoter Diacetyl Newflo-type Flocculation |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-008-9773-7 |
| 通讯作者 | He, xiuping(hexp@sun.im.ac.cn) |
| 英文摘要 | The expression cassette 110 containing the new-found flocculation gene, flons, was transformed into an industrial strain saccharomyces cerevisiae ysf5. upstream activating sequences of the s. cerevisiae alcohol dehydrogenase ii (adh2) gene promoter (p(u-adh2)) were used to regulate the expression of flons; alpha-acetolactate synthase gene ilv2 was chosen for homologous recombination of 110 to the ysf5 chromosome; copper binding metallothionein (encoded by cup1) was used for selection of transformants. ten randomly selected transformants exhibited increased flocculation ability of 1.5 to 2.3 fold more than the original strain. based on their sensitivity to glucose, maltose and sucrose, flocculation property of the transformants was supported to be newflo-type. after successive subculture, the introduced cup1 remained in the transformants. at the end of simulated fermentation test, diacetyl content of the culture media of 5i-1 was 0.45 g l(-1), lower than ysf5 (0.48 g l(-1)). |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; BREWERS-YEAST ; EXPRESSION ; SUGAR |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259412200021 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2384242 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, Xiuping |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Lab Mol Genet & Breeding Yeasts, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dongli,Wang, Zhaoyue,Liu, Nan,et al. Genetic modification of industrial yeast strains to obtain controllable newflo flocculation property and lower diacetyl production[J]. Biotechnology letters,2008,30(11):2013-2018. |
| APA | Wang, Dongli,Wang, Zhaoyue,Liu, Nan,He, Xiuping,&Zhang, Borun.(2008).Genetic modification of industrial yeast strains to obtain controllable newflo flocculation property and lower diacetyl production.Biotechnology letters,30(11),2013-2018. |
| MLA | Wang, Dongli,et al."Genetic modification of industrial yeast strains to obtain controllable newflo flocculation property and lower diacetyl production".Biotechnology letters 30.11(2008):2013-2018. |
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来源：中国科学院大学
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