Comparison of normalization methods with microrna microarray
文献类型：期刊论文
| 作者 | Hua, You-Jia1,2,3; Tu, Kang1,3; Tang, Zhong-Yi1,3; Li, Yi-Xue1; Mao, Hua-Sheng1,2 |
| 刊名 | Genomics
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 92期号:2页码:122-128 |
| 关键词 | Microrna microarray Normalization Print-tip loess |
| ISSN号 | 0888-7543 |
| DOI | 10.1016/j.ygeno.2008.04.002 |
| 通讯作者 | Li, yi-xue(yxli@sibs.ac.cn) |
| 英文摘要 | Micrornas (mirnas) are a group of rnas that play important roles in regulating gene expression and protein translation. in a previous study, we established an oligonucleotide microarray platform to detect mirna expression. because it contained only hundreds of probes, data normalization was difficult. in this study, the microarray data for eight mirnas extracted from inflamed rat dorsal root ganglion (drg) tissue were normalized using 15 methods and compared with the results of real-time polymerase chain reaction. it was found that the mirna microarray data normalized by the print-tip loess method were the most consistent with results from real-time polymerase chain reaction. moreover, the same pattern was also observed in 14 different types of rat tissue. this study compares a variety of normalization methods and will be helpful in the preprocessing of mirna microarray data. crown copyright (c) 2008 published by elsevier inc. all rights reserved. |
| WOS关键词 | CAENORHABDITIS-ELEGANS ; OLIGONUCLEOTIDE MICROARRAY ; RNA ; GENES ; TRANSLATION ; EXPRESSION ; INITIATION ; TISSUES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000257981500007 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2384366 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Yi-Xue |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Ctr Funct Genom,Bioinformat Ctr, Shanghai 200031, Peoples R China 2.Natl Engn Ctr Biochip, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hua, You-Jia,Tu, Kang,Tang, Zhong-Yi,et al. Comparison of normalization methods with microrna microarray[J]. Genomics,2008,92(2):122-128. |
| APA | Hua, You-Jia,Tu, Kang,Tang, Zhong-Yi,Li, Yi-Xue,&Mao, Hua-Sheng.(2008).Comparison of normalization methods with microrna microarray.Genomics,92(2),122-128. |
| MLA | Hua, You-Jia,et al."Comparison of normalization methods with microrna microarray".Genomics 92.2(2008):122-128. |
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来源：中国科学院大学
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