中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Surface plasmon resonance imaging of limited glycoprotein samples

文献类型:期刊论文

作者Liu, Wei1,3; Chen, Yi1; Yan, Mingdi2
刊名Analyst
出版日期2008-09-01
卷号133期号:9页码:1268-1273
ISSN号0003-2654
DOI10.1039/b804235g
通讯作者Chen, yi(chenyi@iccas.ac.cn)
英文摘要A surface plasmon resonance imaging method has been developed for high throughput recognition and determination of low level glycoproteins with limited sample volume at least down to 50 nl. chicken ovalbumin and immunoglobulin g were chosen as model compounds while bovine serum albumin and lysozyme were used as control. each protein, at a concentration of 0.0080-1.0 mg ml(-1), was printed on one gold sensing film, and the films were simultaneously reacted with a probe solution and viewed using a laboratory-built surface plasmon resonance imaging system. the imaging signals were dependent on the concentration and the type of analyte, with a limit of detection down to at least 0.5 ng. the glycoproteins dotted at either 1.0 mg ml(-1) or 0.010 mg ml(-1) were easily differentiated from the non-glycoproteins by reaction with 200 nm concanavalin a (con a), giving a limit of recognition down also to 0.5 ng glycoprotein. this imaging method was hence considered a new tool for analyzing glycoproteins.
WOS关键词PROTEIN-DNA INTERACTIONS ; GLYCOMICS ; ARRAYS ; LECTIN ; CELLS ; IMMOBILIZATION ; IDENTIFICATION ; GLYCOSYLATION ; TECHNOLOGIES ; SPECIFICITY
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
语种英语
WOS记录号WOS:000259449400023
出版者ROYAL SOC CHEMISTRY
URI标识http://www.irgrid.ac.cn/handle/1471x/2384463
专题中国科学院大学
通讯作者Chen, Yi
作者单位1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
2.Portland State Univ, Dept Chem, Portland, OR 97207 USA
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Liu, Wei,Chen, Yi,Yan, Mingdi. Surface plasmon resonance imaging of limited glycoprotein samples[J]. Analyst,2008,133(9):1268-1273.
APA Liu, Wei,Chen, Yi,&Yan, Mingdi.(2008).Surface plasmon resonance imaging of limited glycoprotein samples.Analyst,133(9),1268-1273.
MLA Liu, Wei,et al."Surface plasmon resonance imaging of limited glycoprotein samples".Analyst 133.9(2008):1268-1273.

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来源:中国科学院大学

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