Transgenic analyses of tgif family proteins in drosophila imply their role in cell growth
文献类型：期刊论文
| 作者 | Wang, Yonghua1,2; Wang, Lixia1; Wang, Zhaohui1 |
| 刊名 | Journal of genetics and genomics
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 35期号:8页码:457-465 |
| 关键词 | Tgif Cell size Cell proliferation Transgenic Drosophila melanogaster |
| ISSN号 | 1673-8527 |
| 通讯作者 | Wang, zhaohui(zhwang@genetics.ac.cn) |
| 英文摘要 | Tg-interacting factors (tgifs) belong to a family of tale-homeodomain proteins including tgif, tgif2, and tgif2lx/y (tgif2 like on x or y chromosome) in human. they potentially play important functions in various tissues during development. mutations in tgif are frequently associated with malformation of forebrain and facial structures; tgif2 proteins are over-expressed in many ovarian cancer cell lines; and tgif2lx/y are specifically expressed in adult testis. the molecular functions of these proteins have been investigated mostly in cultured cells. tgif and tgif2 have been found as transcriptional repressors that modulate tgf-beta signaling. however, these findings are far from sufficient to explain their mutant phenotypes or expression patterns, and the functions of tgif2lx/y have never been reported. here we use drosophila as a model system to explore the functions of tgif family proteins in vivo. we observed in fly tissues such as fat body, epithelia, and neuronal cells, that expressing human tgif2 or human tgif2lx generally inhibited cell growth in size and number. co-expressing drosophila myc, cyclin e, or human c-mycs partially rescued the growth inhibition induced by human tgifs, whereas activated insulin pathway signaling did not. taken together, we provide in in vivo evidence for the potential functions of human tgif2 and tgif2lx in growth control. additionally, we confirmed that drosophila tglfs are transcriptional activators by assaying their activities in spermatogenesis. |
| WOS关键词 | TRANSCRIPTIONAL COREPRESSOR TGIF ; FUNCTIONAL-ANALYSIS ; SIGNALING PATHWAY ; GENE ; SPERMATOGENESIS ; PROLIFERATION ; MELANOGASTER ; METABOLISM ; MUTATIONS ; DIVISION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000259761200002 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2384682 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Zhaohui |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yonghua,Wang, Lixia,Wang, Zhaohui. Transgenic analyses of tgif family proteins in drosophila imply their role in cell growth[J]. Journal of genetics and genomics,2008,35(8):457-465. |
| APA | Wang, Yonghua,Wang, Lixia,&Wang, Zhaohui.(2008).Transgenic analyses of tgif family proteins in drosophila imply their role in cell growth.Journal of genetics and genomics,35(8),457-465. |
| MLA | Wang, Yonghua,et al."Transgenic analyses of tgif family proteins in drosophila imply their role in cell growth".Journal of genetics and genomics 35.8(2008):457-465. |
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来源：中国科学院大学
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