Gene manipulation of a heavy metal hyperaccumulator species thlaspi caerulescens l. via agrobacterium-mediated transformation
文献类型：期刊论文
| 作者 | Guan, Zi Qiu1; Chai, Tuan Yao1; Zhang, Yu Xiu2; Xu, Jin1; Wei, Wei1; Han, Lu1; Cong, Lin1 |
| 刊名 | Molecular biotechnology
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 40期号:1页码:77-86 |
| 关键词 | Agrobacterium tumefaciens Thlaspi caerulescens Genetic transformation Hyperaccumulator Phytoremediation |
| ISSN号 | 1073-6085 |
| DOI | 10.1007/s12033-008-9065-4 |
| 通讯作者 | Chai, tuan yao(tychai@gucas.ac.cn) |
| 英文摘要 | Thlaspi caerulescens l. is well known as a zn/cd hyperaccumulator. the genetic manipulation of t. caerulescens through transgenic technology can modify plant features for use in phytoremediation. here, we describe the efficient transformation of t. caerulescens using agrobacterium tumefaciens strain eha105 harboring a binary vector pbi121 with the nptii gene as a selectable marker, the gus gene as a reporter and a foreign catalase gene. based on the optimal concentration of growth regulators, the shoot cluster regeneration system via callus phase provided the basis of the genetic transformation in t. caerulescens. the key variables in transformation were examined, such as co-cultivation period and bacterial suspension density. optimizing factors for t-dna delivery resulted in kanamycin-resistant transgenic shoots with transformation efficiency more than 20%, proven by histochemical gus assay and pcr analysis. southern analysis of nptii and rt-pcr of catalase gene demonstrated that the foreign genes were integrated in the genome of transformed plantlets. moreover, the activity of catalase enzyme in transgenic plants was obviously higher than in wild-type plants. this method offers new prospects for the genetic engineering of this important hyperaccumulator species. |
| WOS关键词 | PLANTS ; REGENERATION ; CADMIUM ; STRATEGIES ; CATALASE ; BIOLOGY ; LEAVES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000258192500009 |
| 出版者 | HUMANA PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2384846 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Tuan Yao |
| 作者单位 | 1.Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China 2.China Univ Mining & Technol Beijing, Sch Chem & Environm Engn, Dept Biol Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, Zi Qiu,Chai, Tuan Yao,Zhang, Yu Xiu,et al. Gene manipulation of a heavy metal hyperaccumulator species thlaspi caerulescens l. via agrobacterium-mediated transformation[J]. Molecular biotechnology,2008,40(1):77-86. |
| APA | Guan, Zi Qiu.,Chai, Tuan Yao.,Zhang, Yu Xiu.,Xu, Jin.,Wei, Wei.,...&Cong, Lin.(2008).Gene manipulation of a heavy metal hyperaccumulator species thlaspi caerulescens l. via agrobacterium-mediated transformation.Molecular biotechnology,40(1),77-86. |
| MLA | Guan, Zi Qiu,et al."Gene manipulation of a heavy metal hyperaccumulator species thlaspi caerulescens l. via agrobacterium-mediated transformation".Molecular biotechnology 40.1(2008):77-86. |
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来源：中国科学院大学
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