Evidence for the binding mode of porphyrins to G-quadruplex DNA
文献类型：期刊论文
| 作者 | Wei, Chunying1; Jia, Guoqing2; Zhou, Jun2; Han, Gaoyi1; Li, Can2 |
| 刊名 | physical chemistry chemical physics
 |
| 出版日期 | 2009 |
| 卷号 | 11期号:20页码:4025-4032 |
| 产权排序 | 2;2 |
| 通讯作者 | 李灿 |
| 英文摘要 | interactions of porphyrin derivatives 5,10,15,20-tetrakis(n-methylpyridinium-4-yl)-21h,23h-porphyrin (tmpyp4) and 5,10,15,20-tetrakis(n-propylpyridinium-4-yl)-21h,23h-porphyrin (tprpyp4) with human telomeric ag(3)(t(2)ag(3))(3) g-quadruplex dnas in 150 mm k(+)-containing buffer in the presence or absence of 40% molecular crowding agent poly(ethylene glycol) (peg 200) were studied by absorption titration fitting and time-resolved fluorescence spectroscopy. the results show that two tmpyp4 (or tprpyp4) molecules bind to antiparallel/parallel hybrid structure of ag(3)(t(2)ag(3))(3) g-quadruplex by end-stacking and outside groove binding modes in the absence of peg. interestingly, in the presence of peg one porphyrin molecule is stacked between two parallel ag(3)(t(2)ag(3))(3) g-quadruplexes to form a sandwich structure, another porphyrin molecule is bound to the groove of the g-quadruplex. the interactions of tmpyp4 with different structures of ag(3)(t(2)ag(3))(3) g-quadruplex are non cooperative, the binding constants of two independent binding sites are 1.07 x 10(6) and 4.42 x 10(8) m(-1) for an antiparallel/parallel hybrid structure of ag(3)(t(2)ag(3))(3), 8.67 x 10(5) and 2.26 x 10(8) m(-1) for parallel-stranded ag(3)(t(2)ag(3))(3) g-quadruplex. conversely, the two binding sites are cooperative for tprpyp4, the apparent association constants are 5.58 x 10(6) and 1.24 x 10(7) m(-1) for parallel-stranded and antiparallel/parallel hybrid structures of ag(3)(t(2)ag(3))(3) g-quadruplex, respectively. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, physical ; physics, atomic, molecular & chemical |
| 研究领域[WOS] | chemistry ; physics |
| 关键词[WOS] | time-resolved fluorescence ; intramolecular g-quadruplex ; g-quartet dna ; cationic porphyrin ; structural basis ; telomeric dna ; crystal-structures ; ligand-binding ; drug design ; complexes |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000266065400026 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102487]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Inst Mol Sci, Taiyuan 030006, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Chunying,Jia, Guoqing,Zhou, Jun,et al. Evidence for the binding mode of porphyrins to G-quadruplex DNA[J]. physical chemistry chemical physics,2009,11(20):4025-4032. |
| APA | Wei, Chunying,Jia, Guoqing,Zhou, Jun,Han, Gaoyi,&Li, Can.(2009).Evidence for the binding mode of porphyrins to G-quadruplex DNA.physical chemistry chemical physics,11(20),4025-4032. |
| MLA | Wei, Chunying,et al."Evidence for the binding mode of porphyrins to G-quadruplex DNA".physical chemistry chemical physics 11.20(2009):4025-4032. |
入库方式： OAI收割
来源：大连化学物理研究所
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