A retrovirus-based system to stably silence gdf-8 expression and enhance myogenic differentiation in human rhabdomyosarcoma cells
文献类型：期刊论文
| 作者 | Yang, Zhuo1,3; Zhangi, Jingwu1; Cong, Haolong1; Huang, Zenghong1; Sun, Lunquan2; Liu, Changmei1; Tien, Po1 |
| 刊名 | Journal of gene medicine
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 10期号:8页码:825-833 |
| ISSN号 | 1099-498X |
| 关键词 | Differentiation Gdf-8 Retrovirus Rhabdomyosarcomas Rnai |
| DOI | 10.1002/jgm.1216 |
| 通讯作者 | Tien, po(tienpo@sun.im.ac.cn) |
| 英文摘要 | Background myostatin, also called gdf-8, a secreted growth and differentiating factor that belongs to the transforming growth factor-beta superfamily, is a known negative regulator of myogenesis in vivo. overexpression of gdf-8 contributes to the lack of differentiation in human rhabdomyosarcoma (rms) cells. we investigated whether a retrovirus-based rna interference (rnai) system against gdf-8 expression in human rms cells would enhance myogenic differentiation. methods a retrovirus-based rnai system was developed that utilized the u6-rna polymerase iii promoter to drive efficient expression and deliver the gdf8-specific short hairpin rnas (shrnas) in human rms cell a204. in this system, the retrovirus vector was integrated into the host cell genome and allowed stable expression of shrnas. gdf-8 expression was determined by real-time polymerase chain reaction and western blotting analysis. an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to determine the cell proliferation. myogenic differentiation markers were monitored by western blotting analysis. cell cycle and apoptosis was determined by propidium iodide staining and analysed in a flow cytometer. results in the sigdf8 a204 cell pools, the levels of both gdf-8 mrna and protein were dramatically reduced by this rnai system. in differentiation conditions, inhibition of myostatin synthesis led to enhanced cell cycle withdrawal, consequently stimulated myogenic differentiation and increased the rate of tumor cell apoptosis. conclusions the results demonstrate that deactivation of myostatin by using retrovirus-based rnai thus may be useful for therapy in rhabdomyosarcomas. copyright (c) 2008 john wiley & sons, ltd. |
| WOS关键词 | BETA SUPERFAMILY MEMBER ; SKELETAL-MUSCLE MASS ; MYOSTATIN GENE ; MAMMALIAN-CELLS ; MDX MICE ; INHIBITION ; GROWTH ; CATTLE ; MUTATIONS ; BLOCKADE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 语种 | 英语 |
| 出版者 | JOHN WILEY & SONS LTD |
| WOS记录号 | WOS:000258558300001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2384932 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tien, Po |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Mol Virol Res Ctr, Beijing 100080, Peoples R China 2.Univ New S Wales, Fac Med, Sydney, NSW, Australia 3.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Zhuo,Zhangi, Jingwu,Cong, Haolong,et al. A retrovirus-based system to stably silence gdf-8 expression and enhance myogenic differentiation in human rhabdomyosarcoma cells[J]. Journal of gene medicine,2008,10(8):825-833. |
| APA | Yang, Zhuo.,Zhangi, Jingwu.,Cong, Haolong.,Huang, Zenghong.,Sun, Lunquan.,...&Tien, Po.(2008).A retrovirus-based system to stably silence gdf-8 expression and enhance myogenic differentiation in human rhabdomyosarcoma cells.Journal of gene medicine,10(8),825-833. |
| MLA | Yang, Zhuo,et al."A retrovirus-based system to stably silence gdf-8 expression and enhance myogenic differentiation in human rhabdomyosarcoma cells".Journal of gene medicine 10.8(2008):825-833. |
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来源：中国科学院大学
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