Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics
文献类型：期刊论文
| 作者 | Zhou, Jun1,2; Wei, Chunying1,3; Jia, Guoqing1,2; Wang, Xiuli1,2; Feng, Zhaochi1; Li, Can1 |
| 刊名 | biochimie
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 91期号:9页码:1104-1111 |
| 关键词 | G-quadruplex Duplex Structural interconversion Molecular crowding |
| 产权排序 | 1;1 |
| 通讯作者 | 李灿 |
| 英文摘要 | the structural interconversion between the g-quadruplex and duplex in vivo is an important subject. in the present study, we used human telomeric dna duplex composed of ggg(ttaggg)(3)/ccc(taaccc)(3) as a model system to investigate its properties under near physiological conditions by spectroscopic methods. circular dichroism and fluorescence spectra demonstrated that g-quadruplex structure can be formed from duplex at near physiological ph (ph 7.4), salt concentration (150 mm k(+)), and temperature (37 degrees c) in the presence of molecular crowding agent peg (400 g/l), whereas the g-quadruplex structure cannot be formed at 25 degrees c in buffer containing 150 mm k(+) in the presence of peg. it is found that the formation rate of g-quadruplex structure depends on the temperature and the concentrations of both peg and k(+). this work suggests that human telomeric g-quadruplex structure may be potentially formed from watson-crick duplex in vivo. (c) 2009 elsevier masson sas. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | resonance energy-transfer ; g-quartet formation ; molecular crowding conditions ; i-motif structures ; binding-protein ; complementary strand ; dna quadruplexes ; beta-subunit ; equilibrium ; competition |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000269654100006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102511]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics[J]. biochimie,2009,91(9):1104-1111. |
| APA | Zhou, Jun,Wei, Chunying,Jia, Guoqing,Wang, Xiuli,Feng, Zhaochi,&Li, Can.(2009).Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics.biochimie,91(9),1104-1111. |
| MLA | Zhou, Jun,et al."Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics".biochimie 91.9(2009):1104-1111. |
入库方式： OAI收割
来源：大连化学物理研究所
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