Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection
文献类型：期刊论文
| 作者 | Liu, Kun1,2; Lin, Yuqing1,2; Xiang, Ling1; Yu, Ping1; Su, Lei1; Mao, Lanqun1 |
| 刊名 | Neurochemistry international
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 52期号:6页码:1247-1255 |
| 关键词 | In vivo microdialysis On-line electrochemical detection Ascorbic acid Cerebral ischemia/reperfusion Striatum |
| ISSN号 | 0197-0186 |
| DOI | 10.1016/j.neuint.2008.01.006 |
| 通讯作者 | Mao, lanqun(lqmao@iccas.ac.cn) |
| 英文摘要 | Information on the change in extracellular ascorbic acid (aa) during the acute period of cerebral ischemia is of great importance in the early therapeutic intervention of the cerebral ischemic injury since aa is known to be involved into most kinds of neurochemical changes in the cerebral ischemia. this study describes a fast and efficient method through integration of in vivo microdialysis with on-line electrochemical detection for continuous monitoring cerebral aa, allowing comparative study of the change in the extracellular aa level in different brain ischemia/reperfusion models. the method exhibits a high specificity for aa measurements, bearing a good tolerance against the fluctuation in the brain anoxia and acidity induced by cerebral ischemia/reperfusion. in the global two-vessel occlusion (2-vo) ischemia model, the striatum aa did not change with statistic significance until 60 min after occlusion and was decreased to be 91 +/- 3% (n = 5, p < 0.05) of the basal level (8.05 +/- 0.23 mu m) at the time point of 60 min after occlusion. in the 2-vo ischemia/reperfusion model, aa remained unchanged during the 10 min of ischemia, and was sharply increased to be 267 +/- 74% (n = 5, p < 0.05) of the basal level after the initial 15 min of reperfusion, and then decreased to be 122 +/- 33% (n = 5, p < 0.05) of the basal level after 50 min of reperfusion. extracellular aa was largely increased after 5 min of left middle cerebral artery occlusion (lmcao) and was then gradually increased to be 257 +/- 49% (n = 5, p < 0.05) of the basal level after 60 min of lmcao ischemia. in the lmcao ischemia/reperfusion model, aa was greatly increased during 10 min of ischemia and then gradually increased to be 309 +/- 69% (it = 5, p < 0.05) of the basal level after the consecutive 50 min of reperfusion. the results demonstrated here may be useful for understanding the neurochemical processes in the acute period of cerebral ischemia and could thus be important for neuroprotective therapeutics for cerebral ischemic injury. (c) 2008 elsevier ltd. all rights reserved. |
| WOS关键词 | CEREBRAL-ARTERY OCCLUSION ; ACUTE ISCHEMIC-STROKE ; OXIDATIVE STRESS ; RAT-BRAIN ; FOREBRAIN ISCHEMIA ; FOCAL ISCHEMIA ; INVIVO ELECTROCHEMISTRY ; DEHYDROASCORBIC ACID ; VITAMIN-C ; STRIATUM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| WOS类目 | Biochemistry & Molecular Biology ; Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000255431200039 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2385138 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Mao, Lanqun |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.CAS, Grad Sch, Beijing 100086, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Kun,Lin, Yuqing,Xiang, Ling,et al. Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection[J]. Neurochemistry international,2008,52(6):1247-1255. |
| APA | Liu, Kun,Lin, Yuqing,Xiang, Ling,Yu, Ping,Su, Lei,&Mao, Lanqun.(2008).Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection.Neurochemistry international,52(6),1247-1255. |
| MLA | Liu, Kun,et al."Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detection".Neurochemistry international 52.6(2008):1247-1255. |
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来源：中国科学院大学
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