Dnazyme-based colorimetric sensing of lead (pb(2+)) using unmodified gold nanoparticle probes
文献类型：期刊论文
| 作者 | Wei, Hui; Li, Bingling; Li, Jing; Dong, Shaojun; Wang, Erkang |
| 刊名 | Nanotechnology
 |
| 出版日期 | 2008-03-05 |
| 卷号 | 19期号:9页码:5 |
| ISSN号 | 0957-4484 |
| DOI | 10.1088/0957-4484/19/9/095501 |
| 通讯作者 | Wei, hui() |
| 英文摘要 | Novel functional oligonucleotides, especially dnazymes with rna-cleavage activity, have been intensively studied due to their potential applications in therapeutics and sensors. taking advantage of the high specificity of 17e dnazyme for pb(2+), highly sensitive and selective fluorescent, electrochemical and colorimetric sensors have been developed for pb2+. in this work, we report a simple, sensitive and label-free 17e dnazyme-based sensor for pb(2+) detection using unmodified gold nanoparticles (gnps) based on the fact that unfolded single-stranded dna could be adsorbed on the citrate protected gnps while double-stranded dna could not. by our method the substrate cleavage by the 17e dnazyme in the presence of pb(2+) could be monitored by color change of gnps, thereby pb(2+) detection was realized. the detection of pb(2+) could be realized within 20 min, with a detection limit of 500 nm. the selectivity of our sensor has been investigated by challenging the sensing system with other divalent metal ions. since common steps such as modification and separation could be successfully avoided, the sensor developed here could provide a simple, cost-effective yet rapid and sensitive measurement tool for pb(2+) detection and may prove useful in the development of sensors for clinical toxicology and environmental monitoring in the future. |
| WOS关键词 | RNA-CLEAVING DEOXYRIBOZYME ; IN-VITRO SELECTION ; SINGLE-STRANDED-DNA ; CATALYTIC DNA ; ELECTROCHEMICAL DETECTION ; APTAMER ; ENZYMES ; BIOSENSOR ; THROMBIN ; SPECTROMETRY |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| 语种 | 英语 |
| WOS记录号 | WOS:000254173900006 |
| 出版者 | IOP PUBLISHING LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2385362 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wei, Hui |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Hui,Li, Bingling,Li, Jing,et al. Dnazyme-based colorimetric sensing of lead (pb(2+)) using unmodified gold nanoparticle probes[J]. Nanotechnology,2008,19(9):5. |
| APA | Wei, Hui,Li, Bingling,Li, Jing,Dong, Shaojun,&Wang, Erkang.(2008).Dnazyme-based colorimetric sensing of lead (pb(2+)) using unmodified gold nanoparticle probes.Nanotechnology,19(9),5. |
| MLA | Wei, Hui,et al."Dnazyme-based colorimetric sensing of lead (pb(2+)) using unmodified gold nanoparticle probes".Nanotechnology 19.9(2008):5. |
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来源：中国科学院大学
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