Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry
文献类型：期刊论文
| 作者 | Cai, Xiaoming1; Zou, Lijuan1,2; Dong, Jun1; Zhao, Lili2; Wang, Yuanyuan2; Xu, Qing1; Xue, Xingya1; Zhang, Xiuli1; Liang, Xinmiao1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2009-09-14 |
| 卷号 | 650期号:1页码:10-15 |
| 关键词 | Hydrophilic interaction liquid chromatography Plasma Metabonomics Highly polar metabolites |
| 产权排序 | 1;1 |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | highly polar metabolites are abundant in human plasma. analysis of these compounds by standard reverse-phase chromatography is difficult. highly polar metabolites were extracted by a two-step method. they were analyzed by ultra-performance hydrophilic interaction chromatography (hilic) coupled to quadrupole-time of flight mass spectrometer detection technology. the middle and low polar fraction were also analyzed using a standard c18 column. data were extracted by markerlynx, and analyzed by multivariate statistical methods to discriminate human plasma samples by sex and age. the results not only showed good classification of samples by sex and age, but also revealed new potential biomarkers not revealed by standard rplc/ms analysis. this study showed that hilic is an effective technique to analyze highly polar metabolites for metabonomic studies. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | multivariate statistical-analysis ; protein precipitation ; rat urine ; metabonomics ; separation ; ms ; spectroscopy ; age |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000270003800003 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102583]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China 2.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Xiaoming,Zou, Lijuan,Dong, Jun,et al. Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. analytica chimica acta,2009,650(1):10-15. |
| APA | Cai, Xiaoming.,Zou, Lijuan.,Dong, Jun.,Zhao, Lili.,Wang, Yuanyuan.,...&Liang, Xinmiao.(2009).Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry.analytica chimica acta,650(1),10-15. |
| MLA | Cai, Xiaoming,et al."Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry".analytica chimica acta 650.1(2009):10-15. |
入库方式： OAI收割
来源：大连化学物理研究所
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