Is kinase activity essential for biological functions of bri1?
文献类型：期刊论文
| 作者 | Xu, Weihui1,2; Huang, Juan1,2,3; Li, Baohua1,2; Li, Jiayang1,2; Wang, Yonghong1,2 |
| 刊名 | Cell research
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 18期号:4页码:472-478 |
| 关键词 | Brassinosteroid Bri1 Kinase activity Arabidopsis thaliana |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2008.36 |
| 通讯作者 | Wang, yonghong(yhwang@genetics.ac.cn) |
| 英文摘要 | Brassinosteroids (brs) are a major group of plant hormones that regulate plant growth and development. bri1, a protein localized to the plasma membrane, functions as a br receptor and it has been proposed that its kinase activity has an essential role in br-regulated plant growth and development. here we report the isolation and molecular characterization of a new allele of bri1, bri1-301, which shows moderate morphological phenotypes and a reduced response to brs under normal growth conditions. sequence analysis identified a two-base alteration from gg to at, resulting in a conversion of 989g to 9891 in the bri1 kinase domain. an in vitro assay of kinase activity showed that bri1-301 has no detectable autophosphorylation activity or phosphorylation activity towards the bri1 substrates ttl and bak1. furthermore, our results suggest that bri1-301, even with extremely impaired kinase activity, still retains partial function in regulating plant growth and development, which raises the question of whether bri1 kinase activity is essential for br-mediated growth and development in higher plants. |
| WOS关键词 | RECEPTOR KINASE ; BRASSINOSTEROID RECEPTORS ; EXTRACELLULAR DOMAIN ; SIGNAL-TRANSDUCTION ; STEROID-HORMONE ; PLASMA-MEMBRANE ; PLANT-GROWTH ; ARABIDOPSIS ; LIGHT ; GENE |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256076100006 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2385423 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Yonghong |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Weihui,Huang, Juan,Li, Baohua,et al. Is kinase activity essential for biological functions of bri1?[J]. Cell research,2008,18(4):472-478. |
| APA | Xu, Weihui,Huang, Juan,Li, Baohua,Li, Jiayang,&Wang, Yonghong.(2008).Is kinase activity essential for biological functions of bri1?.Cell research,18(4),472-478. |
| MLA | Xu, Weihui,et al."Is kinase activity essential for biological functions of bri1?".Cell research 18.4(2008):472-478. |
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来源：中国科学院大学
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