Purification of alkaloids from Corydalis yanhusuo W. T. Wang using preparative 2-D HPLC
文献类型：期刊论文
| 作者 | Zhang, Jing1; Jin, Yu2; Liu, Yanfang1; Mao, Yuansheng1; Feng, Jiatao1; Xue, Xingya1; Zhang, Xiuli1; Liang, Xinmiao1,2 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 32期号:9页码:1401-1406 |
| 关键词 | Alkaloids Corydalis yanhusuo W. T. Wang pH values differences Preparative parallel HPLC Two-dimensional HPLC |
| 产权排序 | 1;1 |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | two-dimensional preparative multi-channel parallel high performance liquid chromatography was successfully applied for the first time to isolate and purify alkaloids from corydalis yanhusuo. the experiments were performed in off-line mode using the same preparative chromatographic column with ph 3.5 in the first and ph 10.0 in the second separation dimension. in the preparative process, uv-triggered fraction collection was used in the first dimension while uv and ms-triggered collection were used in the second dimension for reasons of sensitivity and complementarity. two pure compounds and nine fractions were obtained in the first dimension. then two representative fractions were further purified in the second dimension and six pure compounds were obtained. the results demonstrated that this procedure is an effective approach for the preparative isolation and purification of alkaloids from corydalis yanhusuo. based on the different ph values of the mobile phase in this method, it is also suitable for the preparative isolation and purification of other compounds from tcms which are sensitive to the ph of the solutions. moreover, this method will be a promising tool for the purification of low content compounds from natural products. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; counter-current chromatography ; combinatorial libraries ; column chromatography ; separation ; flavonoids ; system ; leaves ; ms |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000266341200017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102601]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jing,Jin, Yu,Liu, Yanfang,et al. Purification of alkaloids from Corydalis yanhusuo W. T. Wang using preparative 2-D HPLC[J]. journal of separation science,2009,32(9):1401-1406. |
| APA | Zhang, Jing.,Jin, Yu.,Liu, Yanfang.,Mao, Yuansheng.,Feng, Jiatao.,...&Liang, Xinmiao.(2009).Purification of alkaloids from Corydalis yanhusuo W. T. Wang using preparative 2-D HPLC.journal of separation science,32(9),1401-1406. |
| MLA | Zhang, Jing,et al."Purification of alkaloids from Corydalis yanhusuo W. T. Wang using preparative 2-D HPLC".journal of separation science 32.9(2009):1401-1406. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。