Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang
文献类型：期刊论文
| 作者 | Zhang, Jing1; Jin, Yu; Liu, Yanfang1; Xiao, Yuansheng1; Feng, Jiatao1; Xue, Xingya1; Zhang, Xiuli1; Liang, Xinmiao1,2 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 32期号:12页码:2084-2089 |
| 关键词 | Alkaloids Corydalis yanhusuo W. T. Wang Different pH RPLC-RPLC Traditional Chinese medicines |
| 产权排序 | 1;1 |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | an effective method utilizing the same rp chromatographic column with different ph in first and second lc dimensions has been developed for separation of the basic compounds from traditional chinese medicines (tcms). in this work, the alkaloids in corydalis yanhusuo which is an important tcm were selected as a model to develop the method. the additives and ph values of the mobile phase were optimized in this work. to investigate the feasibility of this method, off-line mode separation was performed in the experiments. according to the uv-absorption intensity, there were eight fractions collected in acidic conditions. all the fractions were analyzed in basic conditions. the results showed that the chromatographic selectivities were significantly different in the separations performed with acidic and alkaline elution systems. complementary separation was achieved in this work. it is demonstrated that this method would be an effective tool for alkaloids research. based on the different ph of the mobile phase in this method, it could also be suitable to analyze compounds which were sensible to the ph of the solution. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; electrospray mass-spectrometry ; chinese medicine ; quantitative-determination ; tetrahydropalmatine ; protoberberine ; peptides ; aconitum ; hplc ; ms |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000267811600011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102603]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jing,Jin, Yu,Liu, Yanfang,et al. Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang[J]. journal of separation science,2009,32(12):2084-2089. |
| APA | Zhang, Jing.,Jin, Yu.,Liu, Yanfang.,Xiao, Yuansheng.,Feng, Jiatao.,...&Liang, Xinmiao.(2009).Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang.journal of separation science,32(12),2084-2089. |
| MLA | Zhang, Jing,et al."Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang".journal of separation science 32.12(2009):2084-2089. |
入库方式： OAI收割
来源：大连化学物理研究所
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