Fit interacts with atbhlh38 and atbhlh39 in regulating iron uptake gene expression for iron homeostasis in arabidopsis
文献类型：期刊论文
| 作者 | Yuan, Youxi1,2; Wu, Huilan1; Wang, Ning1,2; Li, Jie1; Zhao, Weina1,2; Du, Juan1,2; Wang, Daowen1; Ling, Hong-Qing1 |
| 刊名 | Cell research
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 18期号:3页码:385-397 |
| 关键词 | Activation of iron uptake genes Arabidipsis thaliana Bhlh transcription factor Iron homeostasis Protein-protein Interaction |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2008.26 |
| 通讯作者 | Ling, hong-qing(hqling@genetics.ac.cn) |
| 英文摘要 | Iron is an essential element for plant growth and development. iron homeostasis in plants is tightly regulated at both transcriptional and posttranscriptional level. several bhlh transcription factors involved in iron homeostasis have been identified recently. however, their regulatory mechanisms remain unknown. in this work, we demonstrate that the transcription factor fit interacted with atbhlh38 and atbhlh39 and directly conferred the expression regulation of iron uptake genes for iron homeostasis in arabidopsis. yeast two-hybrid analysis and transient expression in arabidopsis protoplasts showed that atbhlh38 or atbhlh39 interacted with fit, a central transcription factor involved in iron homeostasis in arabidopsis. expression of fit/atbhlh38 or fit/atbhlh39 in yeast cells activated gus expression driven by ferric chelate reductase (fro2) and ferrous transporter (irt1) promoters. overexpression of fit with either atbhlh38 or atbhlh39 in plants converted the expression of the iron uptake genes fro2 and irt1 from induced to constitutive. further analysis revealed that fro2 and irt1 were not regulated at the posttranscriptional level in these plants because irt1 protein accumulation and high ferric chelate reductase activity were detected in the overexpression plants under both iron deficiency and iron sufficiency. the double overexpression plants accumulated more iron in their shoots than wild type or the plants overexpressing either atbhlh38, atbhlh39 or fit. our data support that ferric-chelate reductase fro2 and ferrous-transporter irt1 are the targets of the three transcription factors and the transcription of fro2 and irt1 is directly regulated by a complex of fit/atbhlh38 or fit/atbhlh39. |
| WOS关键词 | FERRIC-CHELATE REDUCTASE ; TRANSCRIPTION FACTOR FAMILY ; METAL TRANSPORTER ; DIFFERENTIAL REGULATION ; SIGNAL-TRANSDUCTION ; THALIANA ; PLANTS ; TOMATO ; IRT1 ; GROWTH |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000255101000009 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2385512 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ling, Hong-Qing |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Youxi,Wu, Huilan,Wang, Ning,et al. Fit interacts with atbhlh38 and atbhlh39 in regulating iron uptake gene expression for iron homeostasis in arabidopsis[J]. Cell research,2008,18(3):385-397. |
| APA | Yuan, Youxi.,Wu, Huilan.,Wang, Ning.,Li, Jie.,Zhao, Weina.,...&Ling, Hong-Qing.(2008).Fit interacts with atbhlh38 and atbhlh39 in regulating iron uptake gene expression for iron homeostasis in arabidopsis.Cell research,18(3),385-397. |
| MLA | Yuan, Youxi,et al."Fit interacts with atbhlh38 and atbhlh39 in regulating iron uptake gene expression for iron homeostasis in arabidopsis".Cell research 18.3(2008):385-397. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。