Isolation of microsatellite loci in the pollinating fig wasp of ficus hispida, ceratosolen solmsi
文献类型：期刊论文
| 作者 | Yu, Hao1,2; Zhang, Tong-Xin1,2; Hu, Hao-Yuan1,2; Niu, Li-Ming1,2; Xiao, Hui1; Zhang, Yan-Zhou1; Huang, Da-Wei1,3 |
| 刊名 | Insect science
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 15期号:4页码:303-306 |
| 关键词 | Ficus Fig wasp Markers Microsatellite loci Polymorphism Primers |
| ISSN号 | 1672-9609 |
| DOI | 10.1111/j.1744-7917.2008.00214.x |
| 通讯作者 | Huang, da-wei(huangdw@ioz.ac.cn) |
| 英文摘要 | Microsatellite loci were isolated for ceratosolen solmsi, pollinator of the dioecious ficus hispida. we developed nine polymorphic microsatellite loci based on the method of polymerase chain reaction isolation of microsatellite arrays (pima). enrichment of genomic libraries was performed by random amplified polymorphic dna (rapd). a subset of 38 positive clones was sequenced; 15 clones showed microsatellite loci. we tested 15 designed primer pairs and nine of them produced polymorphic amplification in 48 individual wasps collected from different fruits of the dioecious host fig ficus hispida in china. among the 48 individuals, 49 alleles were obtained at the nine loci. the observed heterozygosity ranged between 0.357 and 0.634. |
| WOS关键词 | MARKERS |
| WOS研究方向 | Entomology |
| WOS类目 | Entomology |
| 语种 | 英语 |
| WOS记录号 | WOS:000258095500002 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2385742 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Da-Wei |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China 3.Shandong Agr Univ, Plant Protect Coll, Tai An, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Hao,Zhang, Tong-Xin,Hu, Hao-Yuan,et al. Isolation of microsatellite loci in the pollinating fig wasp of ficus hispida, ceratosolen solmsi[J]. Insect science,2008,15(4):303-306. |
| APA | Yu, Hao.,Zhang, Tong-Xin.,Hu, Hao-Yuan.,Niu, Li-Ming.,Xiao, Hui.,...&Huang, Da-Wei.(2008).Isolation of microsatellite loci in the pollinating fig wasp of ficus hispida, ceratosolen solmsi.Insect science,15(4),303-306. |
| MLA | Yu, Hao,et al."Isolation of microsatellite loci in the pollinating fig wasp of ficus hispida, ceratosolen solmsi".Insect science 15.4(2008):303-306. |
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来源：中国科学院大学
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