Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Ma, Chenfei1; Wang, Huahong2; Lu, Xin1; Wang, Hong2; Xu, Guowang1; Liu, Benye2 |
| 刊名 | metabolomics
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 5期号:4页码:497-506 |
| 关键词 | Amorpha-4 11-Diene synthase Artemisia annua L. Transgenic Terpenoid Metabolic profiling |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | amorpha-4,11-diene synthase (ads) is a very important enzyme which catalyzes the committed step of artemisinin biosynthesis. in this work, two lines of transgenic artemisia annua l. which ads was over-expressed (line a9) and suppressed (line amsi), respectively, were utilized. and the transgenic line gus with beta-glucuronidase gene was regarded as the control. their terpenoid metabolic profiling was investigated by using gc x gc-tofms. the metabolic profiling method established included simple extraction, two-dimension separation and multivariate analysis. partial least squares discriminant analysis (pls-da) was used to classify two transgenic lines and the control line. eleven important compounds in classification were identified. most of them were sesquiterpenoids including monoterpenoid, diterpenoid and four bioprecursors of artemsisnin. compared with the control, artemisinin and bioprecursors in the line a9 increased as a result of over-expressing ads. borneol and phytol also increased in the line a9, but (e)-beta-farnesene and germacrene d were reversely altered. the result indicated that over-expression of the ads affected not only artemisinin biosynthesis, but also the whole metabolic network of terpenoid. compared with the line a9, no opposite change of artemisinin and related derivatives was observed in the line amsi, the ads inhibition had no significant effect on artemisinin biosynthesis in the line amsi. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | endocrinology & metabolism |
| 研究领域[WOS] | endocrinology & metabolism |
| 关键词[WOS] | performance liquid-chromatography ; in-vivo transformations ; antimalarial-drug ; dihydroartemisinic acid ; plants ; biosynthesis ; identification ; synthase ; malaria ; gene |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000272849800009 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102717]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chenfei,Wang, Huahong,Lu, Xin,et al. Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry[J]. metabolomics,2009,5(4):497-506. |
| APA | Ma, Chenfei,Wang, Huahong,Lu, Xin,Wang, Hong,Xu, Guowang,&Liu, Benye.(2009).Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry.metabolomics,5(4),497-506. |
| MLA | Ma, Chenfei,et al."Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry".metabolomics 5.4(2009):497-506. |
入库方式： OAI收割
来源：大连化学物理研究所
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