Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana
文献类型：期刊论文
| 作者 | Guo, Jiangbo1,2; Dai, Xiaojing1,2; Xu, Wenzhong1; Ma, Mi1 |
| 刊名 | Chemosphere
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 72期号:7页码:1020-1026 |
| ISSN号 | 0045-6535 |
| 关键词 | Phytochelatin synthase Glutathione Transgenic Phytoremediation |
| DOI | 10.1016/j.chemosphere.2008.04.018 |
| 通讯作者 | Ma, mi(mami@ibcas.ac.cn) |
| 英文摘要 | The goal of this study was to develop transgenic plants with increased tolerance for and accumulation of heavy metals and metalloids from soil by simultaneous overexpression of aspcs1 and gsh1 (derived from garlic and baker's yeast) in arabidopsis thaliana. phytochelatins (pcs) and glutathione (gsh) are the main binding peptides involved in chelating heavy metal ions in plants and other living organisms. single-gene transgenic lines had higher tolerance to and accumulated more cd and as than wild-type. compared to single-gene transgenic lines, dual-gene transformants exhibited significantly higher tolerance to and accumulated more cd and as. one of the dual-gene transgenic lines, pg1, accumulated twice the amount of cd as single-gene transgenic lines. simultaneous overexpression of aspcs1 and gsh1 led to elevated total pc production in transgenic arabidopsis. these results indicate that such a stacking of modified genes is capable of increasing cd and as tolerance and accumulation in transgenic lines, and represents a highly promising new tool for use in phytoremediation efforts. (c) 2008 elsevier ltd. all rights reserved. |
| WOS关键词 | GAMMA-GLUTAMYLCYSTEINE SYNTHETASE ; HEAVY-METAL DETOXIFICATION ; PHYTOCHELATIN SYNTHASE ; INDIAN MUSTARD ; TOBACCO PLANTS ; EXPRESSION ; CD ; STRESS ; GENES ; ROLES |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS类目 | Environmental Sciences |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000258024300004 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2386289 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, Mi |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Phys, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Jiangbo,Dai, Xiaojing,Xu, Wenzhong,et al. Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana[J]. Chemosphere,2008,72(7):1020-1026. |
| APA | Guo, Jiangbo,Dai, Xiaojing,Xu, Wenzhong,&Ma, Mi.(2008).Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana.Chemosphere,72(7),1020-1026. |
| MLA | Guo, Jiangbo,et al."Overexpressing gsh1 and aspcs1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in arabidopsis thaliana".Chemosphere 72.7(2008):1020-1026. |
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来源：中国科学院大学
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