Glycerol accumulation in the dimorphic yeast saccharomycopsis fibuligera: cloning of two glycerol 3-phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress
文献类型：期刊论文
| 作者 | Yan, Hua1,2; Jia, Liang-Hui1,2; Lin, Yu-Ping1,2; Jiang, Ning1 |
| 刊名 | Yeast
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 25期号:9页码:609-621 |
| 关键词 | Saccharomycopsis fibuligera Glycerol 3-phosphate dehydrogenase (gpd) Isogene Glycerol Osmotic stress Anoxic conditions |
| ISSN号 | 0749-503X |
| DOI | 10.1002/yea.1606 |
| 通讯作者 | Jiang, ning(jiangn@sun.im.ac.cn) |
| 英文摘要 | Glycerol plays an important role in the osmoadaptation responses of saccharomyces cerevisiae. however, there is no detailed investigation about the role of glycerol in the osmoadaptation responses of saccharomycopsis fibuligera. here we show that both intra- and extracellular glycerol concentrations in sm. fibuligera cells responded very quickly when they were subjected to osmotic stress. we then cloned two isogenes encoding putative nad+-dependent glycerol 3-phosphate dehydrogenase (gpd) from sm. fibuligera pd70 by degenerate pcr and subsequent chromosome walking methods. those two genes, designated sfgpd1 and sfgpd2, respectively, exhibited 86.6% pairwise identity in their encoding regions, while there was no obvious homology in their non-coding regions. either sfgpd1 or sfgpd2 could complement the salt tolerance characteristics of the gpd1gpd2 double mutant strain of s. cerevisiae, further demonstrating that both of those genes are functional homologues of s. cerevisiae gpd1 and gpd2. northern blot analysis revealed that sfgpd1 was induced markedly by osmotic stress, while sfgpd2 was not. in consistency with the observation that there was no obvious glycerol content change when the cells were transferred to anoxic conditions, neither sfgpd1 nor sfgpd2 was induced when the cells were transferred to anoxic conditions, thus suggesting a functional splitting of glycerol 3-phosphate dehydrogenase between s. cerevisiae and sm. fibuligera. the genbank accession nos for sfgpd1 and sfgpd2 were dq536095 and dq536096, respectively. copyright (c) 2008 john wiley & sons, ltd. |
| WOS关键词 | SALT-TOLERANT YEAST ; GLYCEROL-3-PHOSPHATE DEHYDROGENASE ; ZYGOSACCHAROMYCES-ROUXII ; DEBARYOMYCES-HANSENII ; REDOX BALANCES ; CEREVISIAE ; OSMOREGULATION ; GROWTH ; DNA ; METABOLISM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259537700001 |
| 出版者 | JOHN WILEY & SONS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2386307 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Ning |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Hua,Jia, Liang-Hui,Lin, Yu-Ping,et al. Glycerol accumulation in the dimorphic yeast saccharomycopsis fibuligera: cloning of two glycerol 3-phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress[J]. Yeast,2008,25(9):609-621. |
| APA | Yan, Hua,Jia, Liang-Hui,Lin, Yu-Ping,&Jiang, Ning.(2008).Glycerol accumulation in the dimorphic yeast saccharomycopsis fibuligera: cloning of two glycerol 3-phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress.Yeast,25(9),609-621. |
| MLA | Yan, Hua,et al."Glycerol accumulation in the dimorphic yeast saccharomycopsis fibuligera: cloning of two glycerol 3-phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress".Yeast 25.9(2008):609-621. |
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来源：中国科学院大学
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