Mechanisms of glucose-induced expression of pancreatic-derived factor in pancreatic beta-cells
文献类型：期刊论文
| 作者 | Wang, Oumei1,2; Cai, Kun1,2; Pang, Shanshan1,2; Wang, Ting1,2; Qi, Dongfei1,2; Zhu, Quanfeng1,2; Ni, Zimei1,2; Le, Yingying1,2 |
| 刊名 | Endocrinology
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 149期号:2页码:672-680 |
| ISSN号 | 0013-7227 |
| DOI | 10.1210/en.2007-0106 |
| 通讯作者 | Le, yingying(yyle@sibs.ac.cn) |
| 英文摘要 | Pancreatic-derived factor (pander) is a cytokine-like peptide highly expressed in pancreatic beta-cells. pander was reported to promote apoptosis of pancreatic beta-cells and secrete in response to glucose. here we explored the effects of glucose on pander expression, and the underlying mechanisms in murine pancreatic beta-cell line min6 and primary islets. our results showed that glucose up-regulated pander mrna and protein levels in a time-and dose-dependent manner in min6 cells and pancreatic islets. in cells expressing camp response element-binding protein (creb) dominant-negative construct, glucose failed to induce pander gene expression and promoter activation. treatment of the cells with calcium chelator [egta, 1,2-bis(o-aminophenoxy) ethane-n, n, n', n'-tetraacetic acid tetra(acetoxymethyl) ester (bapta/ am)], the voltage-dependent ca2+ channel inhibitor (nifedipine), the protein kinase a (pka) inhibitor (h89), the protein kinase c (pkc) inhibitor (go6976), or the mapk kinase 1/2 inhibitor (pd98059), all significantly inhibited glucose-induced pander gene expression and promoter activation. further studies showed that glucose induced creb phosphorylation through ca2+-pka-erk1/2 and ca2+-pkc pathways. thus, the ca2+-pka-erk1/2-creb and ca2+-pkc-creb signaling pathways are involved in glucose-induced pander gene expression. wortmannin(phosphatidylinositol 3-kinase inhibitor), ammonium pyrrolidinedithiocarbamate (nuclear factor-kappa b inhibitor and nonspecific antioxidant), and n-acetylcysteine (antioxidant) were also found to inhibit glucose-induced pander promoter activation and gene expression. because there is no nuclear factor-kappa b binding site in the promoter region of pander gene, these results suggest that phosphatidylinositol 3-kinase and reactive oxygen species be involved in glucose-induced pander gene expression. in conclusion, glucose induces pander gene expression in pancreatic beta-cells through multiple signaling pathways. because pander is expressed by pancreatic beta-cells and in response to glucose in a similar way to those of insulin, pander may be involved in glucose homeostasis. |
| WOS关键词 | INSULIN GENE-TRANSCRIPTION ; GROWTH RESPONSE-1 GENE ; KAPPA-B ACTIVATION ; ENDOTHELIAL-CELLS ; PROMOTER ACTIVITY ; FACTOR PANDER ; FACTOR FAM3B ; PROTEIN ; PHOSPHORYLATION ; PATHWAY |
| WOS研究方向 | Endocrinology & Metabolism |
| WOS类目 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000252506800030 |
| 出版者 | ENDOCRINE SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2386317 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Le, Yingying |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Oumei,Cai, Kun,Pang, Shanshan,et al. Mechanisms of glucose-induced expression of pancreatic-derived factor in pancreatic beta-cells[J]. Endocrinology,2008,149(2):672-680. |
| APA | Wang, Oumei.,Cai, Kun.,Pang, Shanshan.,Wang, Ting.,Qi, Dongfei.,...&Le, Yingying.(2008).Mechanisms of glucose-induced expression of pancreatic-derived factor in pancreatic beta-cells.Endocrinology,149(2),672-680. |
| MLA | Wang, Oumei,et al."Mechanisms of glucose-induced expression of pancreatic-derived factor in pancreatic beta-cells".Endocrinology 149.2(2008):672-680. |
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来源：中国科学院大学
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