Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device
文献类型：期刊论文
| 作者 | Li, Chunyu1,2; Li, Hongjing3; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 30期号:24页码:4270-4276 |
| 关键词 | Capillary action Centrifugal force Microfluidics Reciprocating flow Single-nucleotide mismatches |
| 产权排序 | 1;1 |
| 通讯作者 | 秦建华 ; 林炳承 |
| 英文摘要 | in this study, a compact disc (cd) microfluidic device based on reciprocating flow is developed for the rapid discrimination of single-nucleotide mismatch (snm). this device consists of a pdms cd slab containing eight snm assay units and a glass substrate immobilized with dna probe array. the rapid snm assay is realized by two-step controllable reciprocating flow in the microchannel, resulting from centrifugal force and capillary action as well. a perfect-match probe and three mismatched probes containing a snm at various positions were designed to investigate the ability of this device to discriminate snm. based on the difference between the dissociation kinetics of perfect-match and mismatched duplexes, this cd device was able to discriminate snm within 400 s. moreover, only sub-microliter volume dna samples (350 nl) and washing buffers (350 nl) were required per assay unit. the results indicate that this device has the potential to be used for fast, accurate gene diagnostics, especially for the detection of snps. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | oligonucleotide microarrays ; dna hybridization ; mutations ; allele ; cd ; microchips ; platform ; arrays ; gene ; chip |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000273187800017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102787]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chunyu,Li, Hongjing,Qin, Jianhua,et al. Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device[J]. electrophoresis,2009,30(24):4270-4276. |
| APA | Li, Chunyu,Li, Hongjing,Qin, Jianhua,&Lin, Bingcheng.(2009).Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device.electrophoresis,30(24),4270-4276. |
| MLA | Li, Chunyu,et al."Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device".electrophoresis 30.24(2009):4270-4276. |
入库方式： OAI收割
来源：大连化学物理研究所
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