A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
文献类型：期刊论文
| 作者 | Ma, Hui; Jiang, Lei; Shi, Weiwei; Qin, Jianhua; Lin, Bingcheng |
| 刊名 | biomicrofluidics
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 3期号:4页码:044114-1-044114-8 |
| 关键词 | bioMEMS diseases microchannel flow neurophysiology |
| 产权排序 | 1;1 |
| 通讯作者 | 秦建华 ; 林炳承 |
| 英文摘要 | the soil dwelling nematode caenorhabditis elegans (c. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. in this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual c. elegans was developed. the device consisted of a flow layer and a control layer, which were used for worm manipulation. by activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. it was found that the neurotoxin mpp+ enabled to induce mobility defects and dopaminergic neurons loss in worms. the established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. in addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biophysics ; nanoscience & nanotechnology ; physics, fluids & plasmas |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics ; science & technology - other topics ; physics |
| 关键词[WOS] | caenorhabditis-elegans ; on-chip ; system ; immobilization ; genetics ; neurons |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000273217500014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102791]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Hui,Jiang, Lei,Shi, Weiwei,et al. A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans[J]. biomicrofluidics,2009,3(4):044114-1-044114-8. |
| APA | Ma, Hui,Jiang, Lei,Shi, Weiwei,Qin, Jianhua,&Lin, Bingcheng.(2009).A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans.biomicrofluidics,3(4),044114-1-044114-8. |
| MLA | Ma, Hui,et al."A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans".biomicrofluidics 3.4(2009):044114-1-044114-8. |
入库方式： OAI收割
来源：大连化学物理研究所
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