Preparation and evaluation of the biotinylated polymer coating
文献类型：期刊论文
| 作者 | Lu Tian-Cheng1,2; Deng Chao1,2; Sun Jing1,2; Chen Xue-Si1; Mang Pei-Biao1; Jing Xia-Bin1 |
| 刊名 | Chemical journal of chinese universities-chinese
 |
| 出版日期 | 2008-04-10 |
| 卷号 | 29期号:4页码:837-841 |
| 关键词 | Bioactive coating Biotin Streptavidin Protein adsorption Protein immobilization Immunoassays |
| ISSN号 | 0251-0790 |
| 通讯作者 | Jing xia-bin() |
| 英文摘要 | A novel bioactive polymer coating without nonspecific adsorption of proteins was prepared from a biodegradable amphiphilic triblock copolymer, biotinylated poly (ethylene glycol)-b-poly (l-lactide) -b-poly l-lysine) (peg-pla-pll/biotin) by coating it on a substrate and covering it with a layer of blocking coating consisting of gelatin and poly(n-vinyl pyrrolidone). the bioactive surface was characterized by enzyme linked immunoassay, on the basis of sequential immobilizations of horseradish peroxidase labeled streptavidin, biotinylated mouse globulin, and alkaline phosphatase labeled horse anti mouse igg (h + l). the results showed that the bioactive surface was resistant to nonspecific protein adsorption but allowed specific recognition and combination between biotin and streptavidin, and meanwhile, the combined streptavidin retained its bioactivity. therefore, subsequent protein immobilization can be realized via the reaction of the streptavidin and biotinylated proteins. |
| WOS关键词 | PROTEIN IMMOBILIZATION ; RGD PEPTIDE ; COPOLYMER ; HYDROGELS ; ADHESION |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000255101400038 |
| 出版者 | HIGHER EDUCATION PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2386702 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jing Xia-Bin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu Tian-Cheng,Deng Chao,Sun Jing,et al. Preparation and evaluation of the biotinylated polymer coating[J]. Chemical journal of chinese universities-chinese,2008,29(4):837-841. |
| APA | Lu Tian-Cheng,Deng Chao,Sun Jing,Chen Xue-Si,Mang Pei-Biao,&Jing Xia-Bin.(2008).Preparation and evaluation of the biotinylated polymer coating.Chemical journal of chinese universities-chinese,29(4),837-841. |
| MLA | Lu Tian-Cheng,et al."Preparation and evaluation of the biotinylated polymer coating".Chemical journal of chinese universities-chinese 29.4(2008):837-841. |
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来源：中国科学院大学
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