Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1
文献类型：期刊论文
| 作者 | Elowe, Sabine1; Dulla, Kalyan1; Uldschmid, Andreas1; Li, Xiuling2; Dou, Zhen1; Nigg, Erich A.1,3 |
| 刊名 | journal of cell science
 |
| 出版日期 | 2010 |
| 卷号 | 123期号:1页码:84-94 |
| 关键词 | BubR1 Chromosome congression Kinetochore Mitosis Spindle checkpoint |
| 产权排序 | 2;4 |
| 通讯作者 | sabine elowe |
| 英文摘要 | the bubr1 checkpoint protein performs multiple functions in mitosis. we have carried out a functional analysis of conserved motifs of human bubr1 (also known as bub1b) and demonstrate that spindle assembly checkpoint ( sac) and chromosome attachment functions can be uncoupled from each other. mutation of five proline-directed serine phosphorylation sites, identified in vivo by mass spectrometry, essentially abolishes attachment of chromosomes to the spindle but has no effect on sac functionality. by contrast, mutation of the two conserved ken boxes required for sac function does not impact chromosome congression. interestingly, the contribution of the two ken-box motifs is not equal. cdc20 associates with the n-terminal but not c-terminal ken box, and mutation of the n-terminal ken motif results in more severe acceleration of mitotic timing. moreover, the two ken motifs are not sufficient for maximal binding of cdc20 and apc/c, which also requires sequences in the bubr1 c-terminus. finally, mutation of the glebs motif causes loss of bub3 interaction and mislocalization of bubr1 from the kinetochore; concomitantly, bubr1 phosphorylation as well as sac activity and chromosome congression are impaired, indicating that the glebs motif is strictly required for both major functions of human bubr1. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | cell biology |
| 研究领域[WOS] | cell biology |
| 关键词[WOS] | mitotic checkpoint ; assembly checkpoint ; protein bubr1 ; pseudosubstrate inhibitor ; unattached kinetochores ; alignment ; anaphase ; cdc20 ; mad2 ; mitosis |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000272839800010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/102847]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany 2.Chinese Acad Sci, Dalian Inst Chem Phys, Grp 1803, Dalian 116023, Peoples R China 3.Univ Basel, Biozentrum, CH-4056 Basel, Switzerland |
| 推荐引用方式 GB/T 7714 | Elowe, Sabine,Dulla, Kalyan,Uldschmid, Andreas,et al. Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1[J]. journal of cell science,2010,123(1):84-94. |
| APA | Elowe, Sabine,Dulla, Kalyan,Uldschmid, Andreas,Li, Xiuling,Dou, Zhen,&Nigg, Erich A..(2010).Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1.journal of cell science,123(1),84-94. |
| MLA | Elowe, Sabine,et al."Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1".journal of cell science 123.1(2010):84-94. |
入库方式： OAI收割
来源：大连化学物理研究所
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