Atthic, a gene involved in thiamine biosynthesis in arabidopsis thaliana
文献类型：期刊论文
| 作者 | Kong, Danyu1,2; Zhu, Yuxing1,2; Wu, Huilan1; Cheng, Xudong1,2; Liang, Hui1; Ling, Hong-Qing1 |
| 刊名 | Cell research
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 18期号:5页码:566-576 |
| 关键词 | Arabidopsis E. coli thic Atthic Thiamine Pyrimidine biosynthesis Vitamin b-1 |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2008.35 |
| 通讯作者 | Ling, hong-qing(hqling@genetics.ac.cn) |
| 英文摘要 | Thiamine (vitamin b-1) is an essential compound for organisms. it contains a pyrimidine ring structure and a thiazole ring structure. these two moieties of thiamine are synthesized independently and then coupled together. here we report the molecular characterization of atthic, which is involved in thiamine biosynthesis in arabidopsis. atthic is similar to escherichia coli thic, which is involved in pyrimidine biosynthesis in prokaryotes. heterologous expression of atthic could functionally complement the thic knock-out mutant of e. coli. downregulation of atthic expression by t-dna insertion at its promoter region resulted in a drastic reduction of thiamine content in plants and the knock-down mutant thic1 showed albino (white leaves) and lethal phenotypes under the normal culture conditions. the thic1 mutant could be rescued by supplementation of thiamine and its defect functions could be complemented by expression of atthic cdna. transient expression analysis revealed that the atthic protein targets plastids and chloroplasts. atthic was strongly expressed in leaves, flowers and siliques and the transcription of atthic was downregulated by extrinsic thiamine. in conclusion, atthic is a gene involved in pyrimidine synthesis in the thiamine biosynthesis pathway of arabidopsis, and our results provide some new clues for elucidating the pathway of thiamine biosynthesis in plants. |
| WOS关键词 | ESCHERICHIA-COLI ; SALMONELLA-TYPHIMURIUM ; THIAZOLE MOIETY ; SACCHAROMYCES-CEREVISIAE ; MASS-SPECTROMETRY ; BACILLUS-SUBTILIS ; PISUM-SATIVUM ; SULFUR ATOM ; PROTEIN ; VITAMIN-B1 |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256409000006 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2386754 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ling, Hong-Qing |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kong, Danyu,Zhu, Yuxing,Wu, Huilan,et al. Atthic, a gene involved in thiamine biosynthesis in arabidopsis thaliana[J]. Cell research,2008,18(5):566-576. |
| APA | Kong, Danyu,Zhu, Yuxing,Wu, Huilan,Cheng, Xudong,Liang, Hui,&Ling, Hong-Qing.(2008).Atthic, a gene involved in thiamine biosynthesis in arabidopsis thaliana.Cell research,18(5),566-576. |
| MLA | Kong, Danyu,et al."Atthic, a gene involved in thiamine biosynthesis in arabidopsis thaliana".Cell research 18.5(2008):566-576. |
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来源：中国科学院大学
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