Excision of a selective marker in transgenic rice using a novel cre/loxp system controlled by a floral specific promoter
文献类型：期刊论文
| 作者 | Bai, Xianquan1,2,3; Wang, Qiuyun1,2,4; Chu, Chengcai1,2 |
| 刊名 | Transgenic research
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 17期号:6页码:1035-1043 |
| 关键词 | Osmads45 Floral-specific promoter Cre Loxp Marker-free Rice |
| ISSN号 | 0962-8819 |
| DOI | 10.1007/s11248-008-9182-7 |
| 通讯作者 | Chu, chengcai(ccchu@genetics.ac.cn) |
| 英文摘要 | Based on the cre/loxp system, we have developed a novel marker-free system mediating a direct auto-excision of loxp-flanked marker genes from t(1) transgenic rice without any treatment or further offspring crossing. to achieve this, the floral-specific promoter osmads45 was isolated from rice and the expression patterns of osmads45 promoter was characterised by using the pos45:gus transgenic plants. furthermore, the binary vector with cre recombinase under the control of osmads45 promoter was constructed and introduced into rice by agrobacterium-mediated transformation and transgenic rice plants were generated. southern blot analysis showed that auto-excision of the selective markers occurred in some t(1) progeny of the transgenic plants, suggesting that a high auto-excision frequency can be achieved with our cre/loxp system. this auto-excision strategy provides an efficient way of removing the selectable marker gene from transgenic rice. |
| WOS关键词 | SEPARATE T-DNAS ; SELECTABLE MARKER ; AGROBACTERIUM-TUMEFACIENS ; CRE RECOMBINASE ; HIGHER-PLANTS ; GENE ; TOBACCO ; GENOME ; SEGREGATION ; REMOVAL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260379200002 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2386984 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chu, Chengcai |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Sci Tech Innovat Ctr Hainan State Farm, Haikou 570206, Hainan, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Xianquan,Wang, Qiuyun,Chu, Chengcai. Excision of a selective marker in transgenic rice using a novel cre/loxp system controlled by a floral specific promoter[J]. Transgenic research,2008,17(6):1035-1043. |
| APA | Bai, Xianquan,Wang, Qiuyun,&Chu, Chengcai.(2008).Excision of a selective marker in transgenic rice using a novel cre/loxp system controlled by a floral specific promoter.Transgenic research,17(6),1035-1043. |
| MLA | Bai, Xianquan,et al."Excision of a selective marker in transgenic rice using a novel cre/loxp system controlled by a floral specific promoter".Transgenic research 17.6(2008):1035-1043. |
入库方式： iSwitch采集
来源：中国科学院大学
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